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Information × Registration Number 0223U000203, 0122U001203 , R & D reports Title Molecular-genetic and cellular markers of inflammaging in peripheral blood leukocytes of persons affected as a result of the accident at the Сhernobyl nuclear power plant popup.stage_title Head Bazyka Dymytrii A., Доктор медичних наук Registration Date 05-01-2023 Organization State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine" popup.description2 The object of the study is the peripheral blood leukocytes (PB) of 116 clean-up workers at the Chornobyl nuclear power plant, 65 people of the comparison group and 29 people of the non-irradiated control group. The work aims to investigate indicators of immune status, autophagy, and cellular aging in peripheral blood leukocytes of clean-up workers at the Chornobyl Nuclear Power Plant. The results. According to the research data of the formed analytical sample, similar age-associated changes in immunoreactivity according to the indicators of expression of differential and activation antigens were established in all observation groups. In the clean-up workers and employees of the Chornobyl nuclear power plant, we examined a decrease in the relative level of IL-1β-, IFN-γ-producing CD45+CD14- lymphocytes, spontaneous expression of the LC3B protein in PB leukocytes. A correlation between the level of the LC3B protein was determined and the expression of NF-κB protein in lymphocytes of clean-up workers PB with D < 100 mSv. A decrease in relative telomere length (RTL) and a correlation dependence of NF-κB with radiation dose and RTL were established. These changes can contribute to chronic inflammation and premature cellular aging. Furthermore, LC3B and NF-κB proteins are identified as inflammatory markers. A decrease in RTL, spontaneous expression of the LC3B protein, the level of IL-6-, IL-2-, and IL-1β-producing lymphocytes, and an increase in IL-1β and TNF-α-producing lymphocytes were determined in the group of servicemen of the Armed Forces of Ukraine. A direct correlation was determined between the relative expression levels of NF-κB and LC3B proteins, as well as NF-κB and IL-1β-producing lymphocytes, which is probably associated with the activation of stress-induced NF-κB signal transduction, which can lead to accelerated acquisition by cells of the secretory phenotype associated with cellular senescence (SASP) and the development of chronic mild inflammation. Product Description popup.authors Ilienko Iryna Mykolaivna Belayev Oleg А. Vorobyov Mykola O. Golyarnik Nataliya A. Zvarych Liliia M. Lyaskivska Olena V. Panchenko Viktoriia V. popup.nrat_date 2023-01-05 Close