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Information × Registration Number 0223U000278, 0120U100298 , R & D reports Title Investigation of the role of сell signaling second messengers in action of phytohormones and their structural analogs. popup.stage_title Head Kravets Volodymyr S., Доктор біологічних наук Registration Date 05-01-2023 Organization V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry of the National Academy of Sciences of Ukraine popup.description2 Were obtained results on the effect of peptide flg22 and inactivated phytopathogenic bacteria Pseudomonas syringae on the induction of resistance in Arabidopsis thaliana wild-type plants and transgenic lines with mutations in the genes of phospholipase D in response to infection with active bacteria Pseudomonas syringae by direct inoculation of bacterial cells into the apoplast of leaf blades and by the method of "flood-innoculation". The effect of flg22 peptide on the formation of lipid secondary intermediates in Arabidopsis thaliana plants of wild type and transgenic lines with mutations in the genes of phospholipases D was established. In the process of studying the molecular mechanisms of plant resistance to biotic stress, a number of plant metabolic enzymes, namely glyoxalate/hydroxypyruvate reductase A, glutathione peroxidase 2, glutathione S-transferase F8, were cloned and obtained as recombinant proteins for the first time. Cloning of thioredoxin M1 in the form of recombinant protein with streptavidin tag (strep-tag) was performed. These proteins will be tested for the ability to bind to salicylic acid by isothermal titration calorimetry, which will establish their role in the biological role of salicylic acid in plant cells. Using in vitro test systems, the level of inhibition of root growth of mutant lines of Arabidopsis thaliana plants dgk3.5.6 (triple mutants), dgk1.2 (double mutants), dgk4 and dgk7 with knockout of diacylglycerol kinase genes in the presence of PEP1 peptide was determined. Product Description popup.authors Bulava Semen O. Bukhonska Yaroslava К. Derevyanchuk Michael V. Kolesnikov Yaroslav S. Kretynin Serhiy V. Nikolayevska Valentyna І. Pokotylo Igor V. Yakovenko Oksana М. popup.nrat_date 2023-01-05 Close