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Information × Registration Number 0223U000407, 0121U111965 , R & D reports Title Low-temperature storage of Dunaliella salina microalgae as a powerful producer of natural beta-carotene popup.stage_title Head Chernobai Nadiia A., к.б.н. Registration Date 07-01-2023 Organization Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description2  Research objects: microalgae Dunaliella salina. The purpose of the work: to study the dependence between preservation after cryopreservation and the natural halotolerance of the microalgae Dunaliella salina Teod., to clarify the mechanisms of cryodamage, as well as to create a basis for the implementation of theoretically grounded approaches in the practice of cryopreservation of unicellular microalgae producers of biologically important compounds. Cryobiological, biochemical, biophysical methods (light and fluorescence microscopy, spectrophotometry), cultivation, morphometry, and statistical research methods were used to perform the work. The work carried out and the analysis of the obtained data showed that the change in the composition of the culture medium (reduction in the content of microelements, increase in the concentration of sodium chloride) promotes the directed synthesis of β-carotene in the cells of the microalgae D. salina. A decrease in temperature to 4°С leads to a change in the pigment composition of cells after 24 hours - the synthesis of chlorophyll is inhibited and the synthesis of carotenoids increases. The main temperature range, within which damage to cells of microalgae D. salina probably occurs during cryopreservation, lies in the range from –10 to –25°С, depending on the culture medium. Cells of microalgae D. salina, grown in conditions of lack of trace elements or increased content of sodium chloride, are more resistant to low and ultra-low temperatures. 50% and 75% PVS 1, PVS 2, PVS 3 (plant vitrification solution) significantly lower the crystallization temperature when cooling the suspension of D. salina microalgae cells, and also allow to completely exclude the crystallization phase during the cooling stage, which makes them promising cryoprotectants in the development of methods long-term storage of D. salina microalgae cells. Product Description popup.authors Chernobai Nadiia A. popup.nrat_date 2023-01-07 Close