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Information × Registration Number 0223U000538, 0122U002424 , R & D reports Title Peculiarities of the hippocampal structure and cognitive function in mice under antibiotic-induced gut dysbiosis in the “mother-child” system popup.stage_title Head Skybo Halyna H., Доктор медичних наук Registration Date 11-01-2023 Organization Department for special training National academy of science of Ukraine at Kiev University popup.description2 The I stage of the research project aimed to evaluate the effects of antibiotic use in mice during pregnancy on the memory and hippocampal structure of their young offspring. To do this, pregnant female C67BL/6J mice were divided into 3 groups: (1) control – drank sterile water during the entire gestation; (2) 2-w antibiotics – consumed aqueous solutions of antibiotics amoxicillin (205 mg/kg/day) and azithromycin (51 mg/kg/day) during the 2nd week of pregnancy; (3) 3-w antibiotics – consumed antibiotics during the 3rd week of gestation. 4-week-old offspring mice were studied. Animals were performed behavioral tests (Morris water maze and novel object recognition test), and their hippocampi were analyzed using light-optical, immunofluorescent, and electron microscopic methods. Young mice born to dams that were treated with a mixture of antibiotics during the 2nd and 3rd weeks of gestation were shown to have impaired spatial memory and learning, which was manifested by a statistically significant increase in the time to find the hidden platform during the 2-3 days of testing in Morris water maze, as well as the path length on the 3rd day of testing, compared to control animals. In contrast, the novel object recognition test did not demonstrate notable changes between groups in long-term associative memory. At the structural level, antibiotics use during pregnancy caused a decrease in the number density of the CA1 pyramidal neurons by 8-11% in the offspring’s hippocampus. In addition, animals of the 2-w antibiotics group showed decreased ​​astrocyte cell surface area and astrocytic territories, which may indicate impairment of homeostasis in brain tissue. In turn, the decline of the neurogenesis level was found in the dentate gyrus of young 3-w antibiotics mice, namely a reduction in the integrated density of doublecortin (DCX)-positive cells and the BrdU/DCX ratio by 23% and 44%, respectively, compared to controls. Ultrastructurally, animals of the 3-w antibiotics gr Product Description popup.authors Ilenko Dmitro O. Kovalenko Tetyana М. Ostrovska Galina V. Skybo Galyna Grygoriyivna Chereshinska Anastasiya V. Shepilov Dmitro R. popup.nrat_date 2023-01-11 Close