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Information × Registration Number 0223U000879, 0122U002051 , R & D reports Title The study of cholinergic mechanisms involved in COVID-19 pathogenesis popup.stage_title Head Skok Maryna V., Доктор біологічних наук Registration Date 21-01-2023 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description2 2. COVID-19 is accompanied by severe inflammation and often results in cognitive impairments like depression, intellectual weakness and memory decline. Nicotinic acetylcholine receptors of α7 subtype (α7 nAChRs) regulate the release of pro-inflammatory cytokines and influence cognitive processes. Fragment (674-685) of SARS-CoV-2 spike protein interacts with (179-190) fragment of α7 nAChR. The aim of the present project was to study the effect of fragment (674-685) and of (674-685)-specific antibodies on neuroinflammation. It has been shown that immunization of mice with (674-685) peptide conjugated to hemocyanin caused worsening of episodic memory, the increase of pro-inflammatory cytokines and decrease of α7 nAChR levels in the brain. Similar symptoms have been observed in non-immunized mice injected with immunoglobulins purified from the blood of immunized animals. Injections of α7 nAChR agonist choline prevented the development of pathological symptoms upon immunization with (674-685) peptide. Immunization with (674-685) peptide resulted in formation of (674-685)-specific, as well as α7(179-190)-specific antibodies; the latter could be an anti-idiotypic response to the appearance of (674-685)-specific antibodies and a real cause of α7 nAChR decrease in the brain and memory impairment. The data obtained allow concluding that immune reaction against fragment (674-685) of SARS-CoV-2 spike protein, which stimulates neuroinflammation, the decrease of α7 nAChR content in the brain and episodic memory impairment in mice, can be the reason of memory decline in people with post-COVID syndrome. Correspondingly, choline can be regarded as potential therapeutic tool to prevent such cognitive impairments.  Product Description popup.authors Kalashnyk Olena Mykolaivna Koval Lyudmyla Mykolayivna Lykhmus Olena Yuriyivna popup.nrat_date 2023-01-21 Close