Search academic texts

Information × Registration Number 0223U001554, 0121U108463 , R & D reports Title Improving the diagnosis of hemorrhagic disease in rabbits using the molecular genetic method popup.stage_title Head Krytsa Yana P., Кандидат ветеринарних наук Registration Date 31-01-2023 Organization Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine popup.description2  The analysis of the most suitable targets for the detection of specific fragments of the rabbit hemorrhagic disease virus genome was carried out, the design of the primer set and the optimization of the reaction conditions were carried out for the further creation of the diagnostic set provided for by the tasks of the NDR. The components of the reaction mixture were optimized and the conditions for conducting PCR-RF were selected for the detection of a specific fragment of the rabbit hemorrhagic disease virus genome by real-time PCR. The confidence interval of the analytical sensitivity of the diagnostic kit for the HCC virus of the first and second types is 100%. Thus, the LOD of the validated test system is one copy of the target RNA of the HCC virus of the first and second types. Product Description popup.authors popup.nrat_date 2023-01-31 Close