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Information × Registration Number 0223U002234, 0119U103905 , R & D reports Title Role of mitochondrial protein MRPS18-2 and RB in cell stemness control and carcinogenesis, 2.2.5.428 popup.stage_title Head Kashuba Elena Vitaliyivna, Доктор біологічних наук Registration Date 13-02-2023 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2  The mechanism of development of childhood tumors, that originate from progenitor cells that do not undergo the terminal differentiation, is still unknown. Therefore, it is important to determine the molecular mechanisms that underlie the occurrence of childhood tumors with the aim to develop the personalized treatment. It is known that the cellular RB-E2F1 pathway is inactivated in such tumors. Therefore, the search for proteins that control the abovementioned and other cellular pathways is an important and urgent task of modern oncology. A promising candidate is the MRPS18-2 protein, that interacts with RB and induces primary cell transformation. To achieve our goal, i.e., to find out a role of the MRPS18-2-RB interaction in the control on cell stemness and in development of childhood tumors, the expression pattern of the MRPS18-1-3 and RB1 genes was studied. Gene mRNA levels were assessed by qPCR in clinical samples (blood serum and tumor tissue); protein signals were determined in childhood tumors - glioma and medulloblastoma - by immunohistochemical and western blot analyses. The MRPS18-2 and -3 genes are expressed in tumor tissues at higher levels compared to controls. In addition, the MRPS18-2 protein signal is dramatically increased in medulloblastoma glioma cells compared with healthy brain tissue. Thus, members of the MRPS18 protein family may serve as prognostic markers. Product Description popup.authors Astrid Kateryna V. Kalman Serhiy S Kovalevska Larysa M. Matvieieva Alina S Feshyna Mariia O. Shcherbina Valeriia M. popup.nrat_date 2023-02-13 Close