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Information × Registration Number 0223U002304, 0120U104290 , R & D reports Title Affine sensor system based on "smart" hybrid nanocomplexes for determination of specific nucleic acid sequences". Section 1. Development and optimization of the sensory system. popup.stage_title Head Borkovska Lyudmyla V., Доктор фізико-математичних наук Registration Date 14-02-2023 Organization VE Lashkarev Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine popup.description2 The objects of study - unmodified and modified with oligonucleotides gold nanoparticles (Au NPs) and quantum dots (QDs) of Ag(Cu)-In-S/ZnS(CdS); fluorescein; 80-base target oligonucleotide. The aim of the work is to develop an optical sensor system based on "smart" hybrid nanocomplexes for detecting the bcr-abl hybrid gene in the solutions. Research methods – photoluminescence (PL), X-ray diffraction, optical absorption. The dependencies of PL characteristics of Ag(Cu)-In-S QDs on Ag(Cu) content and the presence of ZnS and CdS shells, as well as the acidity of buffer solution were determined. The influence of modification of QDs and Au NPs with oligonucleotides, the concentration of nano objects and the incubation time of the solutions on the PL of QDs was studied. The effect of quenching of the PL intensity of QDs in the SSC buffer solution by Au NPs surface-modified with thiols was revealed, and its main mechanisms were established: partial absorption of the excitation light by Au NPs and the formation of aggregates from QDs, Au NPs, and oligonucleotides. An optical sensor system which consists of nanoobjects that interact with each other in solution is proposed. The system detects the target oligonucleotide in the solution by registering the changes in the PL intensity of the QDs, which is caused by the interaction of QDs and Au NPs. The possibility of detecting the bcr-abl hybrid gene in the solutions using AgInS2/ZnS QDs and Au NPs has been demonstrated. An algorithm for determining specific nucleic acid sequences in the solutions is proposed. Prospects for future development of the objects of study - improvement of the methods of synthesis of QDs based on A1-A3-B6 compounds with intense PL in the visible spectral range and Au NPs; improvement of the method of registration of specific nucleic acid sequences using QDs based on A1-A3-B6 compounds and Au NPs. Quantum Dot; AgInS2/ZnS, Au Nanoparticle; Fluoresceine, Photoluminescence; Bio-sensor Product Description popup.authors Kozoriz Kostiantyn Stara Tetyana popup.nrat_date 2023-02-14 Close