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Information × Registration Number 0223U002535, 0121U112064 , R & D reports Title Development of antimicrobial drugs with nanopolymer carriers and their use in veterinary medicine. popup.stage_title Head Vlizlo Vasyl V., д.вет.н. Registration Date 22-02-2023 Organization Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv popup.description2  New nanocontainers (nanopolymers) were synthesised as drug carriers and tested for targeted delivery of antibiotics to affected tissues and target cells (microorganisms). A PEG-400 nanopolymer carrier made of polyethylene glycol with a molecular weight of 400 Da was synthesized. The minimum inhibitory concentration (MIC) of the PEGylated antibiotic enrofloxacin was determined for Gram-positive and Gram-negative reference laboratory strains (Escherichia coli ATCC 11105, Staphylococcus aureus ATCC 6538Р) and wild strains (Staphylococcus epidermidis and Enterobacter). It has been proven that intramuscular injections of the PEGylated antibiotic enrofloxacin in rats do not cause changes in their clinical condition, hematopoiesis, hemostasis, non-specific resistance and antioxidant system. Complexes of antibiotics (amoxicillin, doxycycline and oxytetracycline) with polyphosphate ester carriers (P4, P6) were obtained and the content of each component in the formed compounds was determined. The antibiotics, polymers and their complexes were administered in average daily therapeutic doses. It was established that the physiological state and blood parameters of animals were within the physiological ranges after use of polyphosphate ester P4 and its complexes with antibiotics. Antimicrobial study established that complexes of polyphosphate esters with penicillin inhibit the growth of S. aureus, E. coli and P. aeruginosa similarly to the traditional form during cultivation in a solid medium. Amoxicillin in a complex with nanotransporters has a higher antimicrobial effect at a dose 3.5 μg/disc. Oxytetracycline in a complex with nanotransporters effectively suppresses the growth of S. aureus in low doses (2.3 - 3.8 μg/disc). Doxycillin in a complex with nanotransporters P4 and P6 more effectively inhibit the growth of S. aureus than traditional forms of antibiotics at doses 1.25 and 0.95 μg/disc. Product Description popup.authors Vlislo Vasyl Nosova Nataliya Ostapiv Dmytro D. Rusyn Vasyl Slivinska Liubov H. Scherbatiy Andriy popup.nrat_date 2023-02-22 Close