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Information × Registration Number 0223U002679, 0118U007344 , R & D reports Title Investigation of the participation of ion channels in intracellular signaling of the nervous system cells in norm and pathology popup.stage_title Head Lukianets Olena O., Registration Date 01-03-2023 Organization Bogomolets Institute of Physiology of the National Academy of Sciences of Ukraine popup.description2  In the reporting period of year 2021, we studied the involvement of calcium ion channels in changes in calcium concentration under hypercalcemia, as an example of the impact of calcium homeostasis on the viability of hippocampal cells. It was found that under hypercalcemia conditions, the viability of hippocampal neurons decreases, with an increase in the number of necrotic neurons. It was determined that the nonspecific calcium conductance blocker lanthanum retains the ability to enhance impulse activity under conditions of CCCP, i.e., there may be mechanisms for restoring neuronal functionality in case of partial mitochondrial dysfunction. We also studied the processes of activation and desensitization of TRPV1 channels under the influence of capsaicin. It was shown that there is a dependence of the amplitude of calcium transients through the TRPV1 channel on the concentration of external calcium, which is characterized by non-linearity and saturation. The mechanism of starvation in rats was also investigated. It has been shown that the most likely mechanism by which starvation increases the body's response to concomitant stress is the previously described mechanism of CRN-neuron sensitization. In our experiments, in intact cells under hypoxia, a 28% increase in the expression of the Кv1.2 gene was recorded compared to intact cells under normoxia, which is explained by the fact that compensatory mechanisms are activated in cells under stress, since an increase in the expression of Kv1.2 channels leads to an increase in membrane hyperpolarization and, accordingly, a decrease in its excitability. It is concluded that Purkinje neurons are sensitive to hypoxic conditions, and their calcium overload can lead to apoptosis and subsequent death. We found a pronounced cumulative effect of memantine, the drug was well tolerated, improved learning ability and motivational behavioral characteristics, reducing anxiety and fear, which can be generally characterized as an adapt Product Description popup.authors Bogdanova Natalia О. Burlak Mar"yana Р. Vrublevsʹkyy Serhiy V. Ganzha Vita V. Gorbachenko Vasyl А. Lukyanets Olena O. Lukyanets Igor О. Magura Igor S. Melnick Igor V. Petrushenko Mariya O. Petrushenko Olena A. Pohoryela Nelli Н. Rozumna Nataliya M. Sotkis Lyudmyla V. Tarasenko Olexandr М. Tyshchenko Yuliya М. Chentsova Ilona I. Shkril Vyacheslav M. Yavorskyy Volodymyr A. popup.nrat_date 2023-03-01 Close