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Information × Registration Number 0223U002855, 0122U002584 , R & D reports Title Development of new medical products based on biologically active substances and nanomaterials for the prevention and treatment of viral infections popup.stage_title Head Spivak Mykola Ya., д.б.н. Registration Date 13-03-2023 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description2  The object of the research is the basics of targeted synthesis of citrates of stabilized NDCs, their characteristics, toxicity, creation of a gel containing NDCs, research of gels and NDCs on experimental models. The aim and task of the research is the synthesis of biocompatible forms of cerium dioxide nanoparticles (CED), determination of the general toxicity of the active substance of the gel - in vitro and in vivo, dose-dependent effects of CED in the experimental study of acute toxicity by different routes of administration and testing of the dermatotropic gel on experimental material. The basics of targeted synthesis of functional nanodisperse materials based on cerium dioxides are outlined. A protocol for obtaining citrate stabilized NDCs with a negative ζ-potential of the sol has been developed. The physico-chemical, optical and biological properties of NDCs were studied. Monodisperse (≈5 NDCs) were characterized by TEM, UV spectroscopy, and dynamic light scattering (DLS) methods, and their toxicity and genotoxicity were determined. The maximum sustained concentration was determined - CC0 - 3.0 mM, under the conditions of introduction of NDC to L929, EPT, VERO, HEp-2 cell cultures. According to the results of determining the acute toxicity of NDC after intragastric administration to rats, it was proved that NDC belongs to the VI class of toxicity. Drinking "Lohmann Brown" hens with 0.05M NDC for 22 weeks does not affect the biochemical parameters of their blood. The antiviral effect of citrate-stabilized NDCs was revealed - inhibition of reproduction of herpes viruses, surrogate hepatitis C virus (HCV) and A/FM/1/47 (H1N1) virus with a selectivity index ≥16.0, 64, 64, respectively. They show antimicrobial activity against C. albicans UKM U-690, Staphylococcus aureus 8325-4, Escherichia coli UKM B-930. The composition of the base of the hydrophilic gel was chosen to enhance the transdermal penetration of the NDC active substance. An experimental series of gel Product Description popup.authors Babenko Lidia P. Bogdan Mykhajlo M. Bohorad-Kobelska Olena Bubnov Rostyslav B. Vasyliuk Olha M. Ganova Larysa A. Dubova Iryna V. Esaulova Valentyna Zholobak Nadiia M. Kyrychenko Anhelina M. Korol Zvitana Lazarenko Liudmyla M. Surikhina Olha Tymoshok Nataliya O Umarova Oksana popup.nrat_date 2023-03-13 Close