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Information × Registration Number 0223U003134, 0118U000041 , R & D reports Title Development of methods of genetic transformation and creation of a source material of winter wheat, resistant to drought popup.stage_title Head Mykhalska Svitlana I., Кандидат біологічних наук Registration Date 31-03-2023 Organization Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine popup.description2 In order to increase the level of resistance to osmotic stresses of selectively valuable wheat genotypes, the effectiveness of partial suppression of endogenous genes of proline catabolism and additional introduction of genes for its synthesis was studied. The analysis of transgenic wheat plants showed that when trying to increase the content of free proline by suppressing proline dehydrogenase genes, there is a correlation between its content and an increase in the level of resistance to osmotic stress. An increase in the level of proline (relative to the control), as a result of a decrease in the level of transcription of endogenous genes of proline dehydrogenase, is preserved in the next generation of transgenic plants, which gives biotechnological plants the ability to germinate in conditions of osmotic stress, ahead of the growth of plants of the original form. During the selection of T-1 generation of wheat, seeds of transgenic plants were selected, which germinated in conditions of chloride (1% NaCl), sulfate (1.2% Na2SO4) salinities and conditions of simulated dehydration (0.5M mannitol). Under conditions of moisture deficit, biotechnological plants T1 and T2 of winter wheat were characterized by better indicators of crop structure compared to the original form. They had a greater mass of grain from the main ear, mass of grain from a plant and mass of a thousand grains. The regularities of accumulation/loss of osmoprotectants both under normal growing conditions and under the effects of osmotic stress were revealed. It is experimentally substantiated that the osmotolerance of transgenic plants is the result of an increase in the level of free proline, the accumulation of which under normal conditions prevails in the leaves, and under the action of stress factors, it is deposited in the root system. It was found that under conditions of osmotic stress, the content of carbohydrates and proteins does not increase in transformed plants against the background of  Product Description popup.authors Bronnikova Larisa Ivanivna Komisarenko Alla Grugorivna Sergeeva Larisa Eugenievna popup.nrat_date 2023-03-31 Close