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Information × Registration Number 0223U003233, 0118U000041 , R & D reports Title Development of methods of genetic transformation and creation of a source material of winter wheat, resistant to drought popup.stage_title Head Mykhalska Svitlana I., Кандидат біологічних наук Registration Date 06-04-2023 Organization Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine popup.description2 Seed generations T1-T3 of genetically modified wheat plants with introduced elements forming a double-stranded RNA suppressor of the proline dehydrogenase gene and an additional copy of the ornithine aminotransferase gene were obtained. The transgenic status of the seed generation of biotechnological wheat plants was confirmed by molecular genetic analysis and by physiological and biochemical indicators of resistance. The analysis of genetically modified wheat plants showed that the increase in the level of proline (relative to the control), as a result of the decrease in the level of transcription of endogenous proline dehydrogenase genes, is preserved in the next generation of transgenic plants. At the same time, partial suppression of the proline dehydrogenase gene causes an increase in yield indicators in drought conditions due to an increase in the content of free L-proline. A significant advantage of biotechnological forms was observed in the mass of grain from the main ear and the mass of grain from the plant, which is the result of the productivity of additional shoots. Under conditions of water deficit, differences were noted in the growth and biomass accumulation of shoots and roots of wheat seedlings with a functional transgene. It is shown that in conditions of osmotic stress in transformed wheat plants against the background of increased proline level, there is no increase in protein content, while adaptive processes in control variants are ensured by their accumulation. It was established that T1-T2 wheat plants with an additional copy of the ornithine-δ-aminotransferase gene are characterized by increased activity of the OAT enzyme, which is not reflected in the content of free L-proline. At the same time, strengthening the expression of the oat gene in T2 causes an improvement in growth indicators, which positively affects the productivity of plants under conditions of water deficit. Product Description popup.authors Bronnikova Larisa Ivanivna Komisarenko Alla Hryhorivna Sergeeva Larisa Yevgenivna popup.nrat_date 2023-04-06 Close