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Information × Registration Number 0223U003704, 0118U003663 , R & D reports Title Study of molecular biological and phenotypic manifestation of transmitted genes performance and their inheritance in biotechnological plants popup.stage_title Head Morgun Bogdan V., Кандидат біологічних наук Registration Date 17-06-2023 Organization Institute of Cell Biology and Genetic Engineering popup.description2 The objects were nptII, S65Tpgfp genes transferred to wheat and corn plants, the stomatal apparatus of common wheat, sphaeroccocum wheat, spelt, and cybrids of the Brassicaceae family. The aim was to study the possibility of transferring foreign genes of various origins into economically valuable plants, to find out the features of their expression and the inheritance of transgenes in a number of generations of common wheat; study of physiological-biochemical and molecular-biological manifestation of traits determined by nuclear and chloroplast genomes in cybrid plants of the Brassicaceae family; study of the morphological features of the stomatal apparatus of common wheat, sphaeroccocum wheat and spelt for the selection of drought-tolerant forms. The inheritance of transgenes (nptII) in the T3 generation of Podolyanka common wheat after Agrobacterium-mediated transformation in planta was studied. The conditions for analyzing transgenic corn for the presence of the S65Tpgfp mutant gene by PCR were optimized. To select the most drought-resistant plants, a study and comparison of the morphological features of the stomatal apparatus of common wheat, sphaeroccocum wheat and spelt was carried out. A genetic construct for editing the wheat genome has been prepared. A collection of petite mutants of the yeast Saccharomyces cerevisiae and S. paradoxus from laboratory, natural and commercial strains was created; a rho0 mutant of S. cerevisiae strain SK1 was studied, in which the stress caused by starvation leads to a significant complication of the structure of biofilms and their fragmentation. It was established that in the petite mutants of S. cerevisiae and S. paradoxus, the invasion of the nutrient medium is inhibited, while the ability to form aggregate invasive growth is preserved. At the molecular level, a high degree of polymorphism was detected in the SC8132X microsatellite locus in the genome of S. cerevisiae strains. Product Description popup.authors Bannykova Mariia O Nitovska Iryna O. Oliinyk Maryna Ye. Panasenko Artem S. Pronina Olha Volodymyrivna Rymar Yuliia Yu popup.nrat_date 2023-06-17 Close