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Registration Number

0223U004082, 0123U102683 , R & D reports

Title

New mechanisms of interaction of plasmonic nanoparticles with potential-dependent ion channels of cells

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Head

Dzhahan Volodymyr M., Доктор фізико-математичних наук

Registration Date

30-08-2023

Organization

V. Lashkarev Institute of Semiconductor Physics of National Academy of Sciences of Ukraine

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 Several series of samples of gold nanoparticles (NPs) of different sizes were obtained by the method of colloidal synthesis in aqueous solutions. To achieve the different size, one of the key parameters of the synthesis was varied, such as the type or concentration of the stabilizer molecule and the concentration or type of the reducing molecule. The obtained samples were characterized by the methods of optical absorption spectroscopy, dynamic light scattering and scanning electron microscopy. A study of their colloidal stability and the reproducibility of the spectral features of the plasmon resonance band in the optical absorption spectra was carried out on solutions of NPs with different sizes. Stability studies were carried out both for the original colloidal solutions and for NPs introduced into a biocompatible buffer, which is directly used for the introduction of NPs into muscle tissue. The obtained NP samples of different sizes were used to study the effect of nanoparticles on the activity of ion channels. In order to confirm that it is NPs that cause the effect on ion channels, studies were conducted in parallel with solutions of molecules that are used as NP stabilizers in the colloid. An experimental verification of the assumption that the effect is due to the plasmon resonance in the NPs, and not to the chemical or mechanical action of the NPs themselves, was performed. For this purpose, the experiments were carried out both with optical (laser) irradiation, which provided resonant excitation of plasmons, and with light irradiation with a wavelength that does not cause such excitation. Control measurements of muscle activity were also performed in the absence of laser radiation.

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2023-08-30