Search academic texts

Registration Number

0223U004702, 0123U102859 , R & D reports

Title

Development of an enzyme kit and a portable biosensor for express assay of creatinine – a marker of acute renal dysfunction

popup.stage_title

Head

Honchar Mykhailo V., д.б.н.

Registration Date

30-11-2023

Organization

Institute of Cell Biology of the National Academy of Sciences of Ukraine

popup.description2

Report on scientific research work: 100 pages, 5 tables, 42 figures, 1 appendice, 82 sources. CREATININE DEIMINASE, CREATININE, NANOMATERIALS, CHEMOENZYMATIC ANALYSIS, STRUCTURAL AND FUNCTIONAL PROPERTIES, PH-SENSITIVE FIELD TRANSISTOR, SILICON OXIDE/NITRIDE The object of the research – creatinine deiminase and creatinine. Subject of the research – biosensors and enzymatic approaches for determination of creatinine. The purpose of the work - the development of new bioanalytical methods for quantitative determination of creatinine - an important biomarker of the functional state of the kidneys. Research methods: chemical, physicochemical, biochemical, microbiological, enzymatic, electron microscopy, X-ray spectral microanalysis, UV-visible spectroscopy, nuclear magnetic resonance spectroscopy, Raman spectroscopy, potentiometric method, enzyme immobilization methods, statistical analysis. Results and novelty: For the first time: – the codaA gene of Corynobacterium glutamicum was cloned in the cells of the parental strain and the activity of CDI in the obtained transformants was investigated; - an enzymatic method of creatinine analysis was created for detection of N-methylhydantoin, the final product of creatinine hydrolysis; - transition metal nanoparticles capable of forming a redox-active complex with N-methylhydantoin were synthesized; - an amperometric chemosensor was developed for the analysis of N-methylhydantoin using nCoCu nanoparticles; - pH-sensitivity, stability of transducers based on pH-sensitive field-effect transistors and their functionality were investigated. Improved: – the method of one-step purification of creatinine deiminase by the use of metal affinity chromatography; – the methodology for obtaining NPs of noble, transition and rare metals, as well as their hybrid forms by methods of chemical reduction of the corresponding salts; – the method of immobilization of creatinine deiminase on the surface of electrochemical transducers.

Product Description

popup.authors

Mykhailo V. Gonchar
Demkiv Olha M.
Zakalska Oksana М.
Zakalskiy Andriy Ye.
Serkiz Roman
Stasiuk Nataliia Ye.

popup.nrat_date

2023-11-30