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Information × Registration Number 0223U004722, 0123U102907 , R & D reports Title The influence of PM preparations suspended in water of anthropogenic smoke on the microbiotic composition of the intestine, analysis of glutamate and GABA signaling in intestinal cells in normal and inflamed conditions, the integrity of the intestinal mucosa and the inflammatory reaction. Comparative analysis of the mucosa and intestinal microbiome in patients with IBS and UC from polluted and agricultural regions. popup.stage_title Head Tolstanova Hanna М., Доктор біологічних наук Registration Date 30-11-2023 Organization Taras Shevchenko National University of Kyiv popup.description2  The object of research is the integrative integrity of the intestinal barrier, the "gut-brain" interaction. The work aims to study the effect of carbon-containing particulate matter of natural and anthropogenic origin on the state of the barrier function of the mucous membrane of the large intestine of rats in normal and pathological conditions, the metabolic activity of the microbiota and glutamate/GABA neurotransmission, and a comparative analysis of the course of diseases in patients with inflammatory diseases intestines of different regions of Ukraine. Research methods: bacteriological, cytological, chemical-analytical, immunological, electrophysiological, physiological, spectrophotometric, biochemical, and clinical methods. Research results: For the first time, the systemic impact of particulate matter derived from the combustion of various materials of both natural and anthropogenic origin on the intestinal barrier function has been systematically investigated. The toxicity level of particulate matter obtained from the combustion of 3 species of wood and 2 types of wood components was determined in vitro and ex vivo. The complex effect of particulate matter of natural and anthropogenic origin on the intestine was established, demonstrating their ability to disrupt the integrity of the intestinal barrier. The study confirmed the dependency of their impact mechanism on the route of introduction into the organism. Clear connections were identified between the origin, dosage, and duration of action of particulate matter and their effect on the intestine. The study revealed the capacity of particulate matter to disrupt the intestinal barrier function by reducing the glycan portion of the mucosal membrane, altering the composition of the intestinal microbiota, increasing paracellular permeability, disrupting ion transport, and enhancing immune response. It was proven that solid nanoparticles have the capability to increase susceptibility to colitis development an Product Description popup.authors Dorofeyev Andriy E. Dorofeeva Hanna A Korbush Mariya Yu. popup.nrat_date 2023-11-30 Close