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Information × Registration Number 0223U005262, 0119U100821 , R & D reports Title Endogenous factors for the development of myeloprpliferative tumors and prostate cancers popup.stage_title Head Telegeev Gennadiy D., Доктор біологічних наук Registration Date 19-12-2023 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  The subject of research: molecular mechanisms of BCR-ABL functioning, the role of Bcr domains in the functioning of normal cells and cells transformed by the BCR-ABL chimeric oncogene, the role of transcription factors in blast crisis, the development of drug resistance in prostate tumors. Methods: Standard molecular biological protocols, which included: isolation of RNA and DNA, oligonucleotide primers design, reverse transcriptase polymerase chain reaction (RT-PCR), PCR, real-time PCR, creation of recombinant DNA, Southern hybridization, DNA sequencing, fluorescence confocal microscopy of live and fixed mammalian cells, ultra-high-resolution fluorescence microscopy STED, etc. Work results.Vitamin E in K562 cells suppresses the expression of the transcription factor Snail1, the main inducer of EMT, and activates the expression of the transcription factor C/EBP alpha - the main regulator of myelopoiesis. It was established that the formation of a protein complex between Bcr-Abl/GLG1 largely depends on the tyrosine kinase activity of the oncoprotein, the violation of which is accompanied by the absence of colocalization between the Bcr-Abl oncoprotein and the GLG1 protein in chronic myeloid leukemia cells. Product Description popup.authors Antonenko Svitlana Vasylivna Gurianov Dmytro Sergiyovych Polischuk Lev Oleksandrovych Shvachko Ludmila P. popup.nrat_date 2023-12-19 Close