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Information × Registration Number 0223U005539, 0120U104870 , R & D reports Title Effects of stress factors on prion-like proteins synthesis in plants popup.stage_title Head Rashydov Namik M., Registration Date 25-12-2023 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description2  The object of the research is proteins with prion-like and amyloid-like properties in plants. The research subject is the induction of prion-like and amyloid-like proteins in plants under the influence of abiotic stressors. The work aims to investigate the mechanisms and features of the formation of amyloidogenic and prion-like proteins in plants under the influence of various stress factors. Research methods: qPCR, Raman- and FTIR-spectroscopy, protein and free amino acid isolation methods, 2D protein electrophoresis in PAAG, UHPLC-mass spectrometry, gel-free mass spectrometry, fluorescence microscopy, bioinformatics and statistics methods. Results and their novelty: FTIR and Raman spectra of plant samples of arabidopsis, flax, soybean, watercress, mint, poplar, willow, chickpea, Parrotia persica, barley, wheat, and pea under the influence of various stress factors were analyzed. It was determined that under the influence of stress factors, the transition of proteins from the α-state to the β-structure is observed in various tissues, the changes accumulate transgenerationally and are correlated with the activity of genes involved in the stress response. A detergent-resistant fraction of amyloid proteins was isolated from Pisum sativum seedlings irradiated with ionizing radiation, and 62 prion-like proteins were identified using exploratory proteomic analysis and the AlphaFold, PLAAC, and STRING databases. Proteins from the leaves of pea seedlings were isolated to determine the content of β-amyloid aggregates using Congo red staining. Analysis of these slides under a fluorescent microscope showed the accumulation of amyloidogenic proteins after the action of ionizing radiation. Proteomic and FTIR spectroscopic studies have demonstrated the presence of «transgenerational memory» in plants associated with prion properties of proteins. Product Description popup.authors popup.nrat_date 2023-12-25 Close