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Information × Registration Number 0224U000186, 0120U103302 , R & D reports Title Study of the mechanisms of bone marrow mesenchymal stromal cells therapeutic effect in rats with radiation skin lesions in allogenic and xenogenic models popup.stage_title Head Uzlenkova Nataliia Ye., Кандидат біологічних наук Registration Date 03-01-2024 Organization State Organization "Grigoriev Institute for Medical Radiologiy and Oncology of the National Academy of Medical Sciences of Ukraine" popup.description2  Object of research: therapeutic efficacy of bone marrow mesenchymal stromal cells (BM MSCs) in skin radiation damages. Purpose: to define the effectiveness and therapeutic activity mechanisms BM MSCs in skin radiation damages in rats on allogeneic and xenogeneic models. Methods: radiobiological, cell culture, morphological, statistical. Equipment: X-ray machine Faxitron MultiRad 225, CO2 incubator ES-160, centrifuge NF 800, microscope Leica DM IL LED, microscope Axiolab 94D 2601, biochemical photoelectric automatic analyzer Respons 910. Theoretical and practical results: the mechanisms of regenerative and anti-inflammatory therapeutic effects of cultured/cryopreserved BM MSCs were established, where one of the important links is the mobilization of BM by allogeneic BM MSCs and the reduction of the endogenous pool of colony-forming (clonogenic) MSCs. Restorative mechanisms were determined by a decrease in the area of the affected areas with accelerated skin healing without the occurrence of chronic ulcers (50 Gy), anti-inflammatory mechanisms - by a more than 2-fold decrease in the level of C-reactive protein in the blood serum. The practical results consisted in proving the therapeutic efficacy and substantiation of the directions of use of ex vivo cultured and cryopreserved/recultivated ex vivo allogeneic and xenogeneic BM MSCs depending on the severity (acute or chronic) of radiation damage to the skin, the method of administration (local injection or systemic transplantation) and the dosage of BM MSCs. Novelty: for the first time on the developed model of radiation skin damage the mechanisms of the therapeutic effect аnd rationale for use cultivated ex vivo and cryopreserved/recultivated ex vivo allogeneic and xenogeneic ВМ MSCs were determined and assessment depending on the damage severity, the method of administration and dosage of BM MSCs Use area – radiobiology. Product Description popup.authors Akimova Olena Olegivna Danylyuk Svitlana Volodymyrivna Leonova Iryna Oleksandrivna Mamatkulova Tetiana Mykolaivna Maslennikova Olena Leonidivna Neniukova Olena Viktorivna Pavlichenko Yuliana Valeriivna Skorobogatova Nataliya Grygorivna popup.nrat_date 2024-01-03 Close