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Information × Registration Number 0224U000578, 0122U002081 , R & D reports Title Study of the reactive microenvironment as a factor in the progression of prostate cancer (2.2.5.453) popup.stage_title Head Zadvornyi Taras V., Кандидат біологічних наук Registration Date 09-01-2024 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2 Using bioinformatics methods, the GSE76260 and GSE202736 datasets were analyzed and a panel of tissue-specific miRNAs associated with the risk of progression of PCa was identified. The role of miR-7-5p, miR-19a-3p, miR-23b-3p, miR-30a-3p, miR-30e-3p, miR-106a-5p in the formation of the reactive microenvironment of the PCa has been proven. It was established that the levels of miR-19a-3p and miR-23b-3p were 4.93 (p<0.01) and 8.97 (p<0.05) times higher in the PCa tissue of patients with a high risk of tumor progression, respectively, compared to similar indicators in the group of patients with a low risk of progression. It was determined that the indicators of miR-7-5p and miR-19a-3p in the tissue of PCa were directly correlated with the level of expression of markers of tumor-associated fibroblasts α-SMA (r=0.49, p<0.05 and r=0.45, p<0, 05, respectively) and VIM (r=0.45, p<0.05 and r=0.46, p<0.05, respectively). The existence of a direct correlation (r=0.44, p=0.0446) between miR-7-5p expression and the degree of infiltration of the prostate gland tissue by tumor-associated macrophages was revealed. The study of the features of TGFB1 expression made it possible to establish that the expression indicators of this gene in the tissue of patients with a high risk of progression were 1.8 times (p<0.05) and 2.2 times (p<0.05) higher at the protein level and mRNA, respectively, compared to tumor tissue of patients with a low risk of progression. Product Description popup.authors Borikun Tetiana V. Mushii Oleksandr M. Pavlova Anna O. popup.nrat_date 2024-01-09 Close