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Information × Registration Number 0224U000608, 0123U100247 , R & D reports Title Study of the effect of introduced genes of proline metabolism on drought resistance and economic value traits of seed generations of winter wheat biotechnological plants popup.stage_title Head Mykhalska Svitlana I., Кандидат біологічних наук Registration Date 10-01-2024 Organization Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine popup.description2 The report contains 54 pages, 5 figures, 12 tables, and 51 literary sources. Object of study. Seed generations (T1 and T4) of biotechnological plants of soft winter wheat (Triticum aestivum L.). with integrated genes of proline metabolism (Pro). The goal of the work. To investigate the level of tolerance to water deficit of T1 and T4 plants of genetically modified winter wheat with partially suppressed expression of the proline dehydrogenase (ProDH) gene and an additional copy of ornithine aminotransferase Research methods. Isolation of native plant DNA, DNA PCR analysis, DNA electrophoresis, determination of the content of proline, carbohydrates, proline dehydrogenase and ornithine aminotransferase enzyme activity, analysis of crop structure indicators, methods of mathematical processing of research results. Results and their novelty. It is shown that genetic regulation of proline synthesis and catabolism processes by reducing the transcription level of endogenous proline dehydrogenase genes and enhancing the expression of the ornithine-δ-aminotransferase gene contributes to increasing the resistance of wheat seed generations to osmotic stresses. It was established that in T1-T4 wheat plants with a double-stranded RNA suppressor of the pdh gene, the activity of proline dehydrogenase under normal watering conditions was lower, compared to the original forms. At the same time, biotechnological plants accumulated more proline than the original genotypes, the level of which, under optimal cultivation conditions, exceeded the original forms by an average of 1.8 times, and under the effects of water deficit, its content increased by 2.3 times, in relation to normal water supply. Adaptation properties of genetically modified plants to water deficit conditions, due to the increased level of free proline, contributed to maintaining the balance of the carbohydrate composition, improving photosynthetic parameters and generally reducing the level of sensitivity. Product Description popup.authors Bronnikova Larisa Ivanivna Komisarenko Alla Hryhorivna Mykhalskyi Leonid O. popup.nrat_date 2024-01-10 Close