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Information × Registration Number 0224U000653, 0119U002512 , R & D reports Title The interaction of the hemostasis system components at the cellular and molecular level in the process of formation and elimination of a thrombus. popup.stage_title Head Volodymyr O. Chernyshenko, Доктор біологічних наук Registration Date 10-01-2024 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description2 It has been predicted in silico, synthesized and tested in vitro effective urokinase inhibitors. Their ability to suppress the proliferation of tumor cells in culture is shown. The proposed compounds are potential non-immunogenic and effective agents with antithrombotic and antimetastatic effects. The participation of the BβN domain in the intermolecular interactions of fibrin(ogen), fibrin polymerization, and formation of the three-dimensional clot base has been experimentally proven. Approbation of disintegrin from the poison of the polychaete as an inhibitor of platelet aggregation in individuals with increased procoagulant potential ex vivo proved that it is capable of inhibiting platelet aggregation in patients with aspirin resistance. Such properties make it possible to consider it as a prototype of a new antiplatelet drug. The combination of turbidimetry and transmission electron microscopy made it possible to demonstrate the difference in the effect of antibodies III-1D and I-4A, which have epitopes within the superhelical part of the D-region of fibrin(ogen), on thrombin-induced polymerization of fibrin. It was found that the acceleration of fibrin polymerization in the presence of the I-4A antibody is the result of the formation of an abnormal fibrin network with areas of different density and a pronounced alternation of knot-like branches and unbranched thin fibrils. At the same time, slowing of fibrin polymerization in the presence of III-1D antibody is not associated with structural changes in the fibrin polymer network. Diagnostic approaches to the study of the coagulation component of hemostasis have been expanded: new kinetic parameters have been proposed that characterize the balance between the processes of formation and elimination of a fibrin clot in human blood plasma. Product Description popup.authors Iskandarov Ruslan Agapov Andriy Igorovych Baydakova Kateryna Vitaliivna Bereznytskiy Gergiy Kostyantynovych Vartanov Volodymyr Grygorovych Gogolinska Genrieta Kazimirivna Hornytska Olha V. Hrabovskyi Oleksii Olehovych Gryshchuk Volodymyr Ivanovych Gryshchuk Olena Oleksandrivna Gudz Egor Anatolievich Druzyna Nadiya Mukolaivna Klimenko Katerina Petrivna Korolova Dar'ya Sergiyvna Kostychenko Olena Petrivna Kurchenko Tetyana Olexandrivna Kucheryaviy Evgen Petrovich Lugovska Nataliia Eduardivna Makogonenko Yevgen Mitrofanovich Marunich Rostyslav Yriyovich Pavlenko Anastasiya Oleksandrivna Pyrogova Liudmyla Vitaliivna Platonov Oleg Maksymovych Platonova Tetyana M. Savchenko Kateryna Sergiivna Slominskiy Oleksandr Yuriyovych Stohnii Yevhen M. Udovenko Anastasiya Vadymivna Chernyshenko Tamara Martynivna Chernyshov Volodymyr Ivanovych popup.nrat_date 2024-01-10 Close