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Information × Registration Number 0224U000745, 0123U103339 , R & D reports Title Impact of bacteriophage-derived dsRNA on macrophages in context of COVID-19 pandemic popup.stage_title Head Skivka Larysa M., Доктор біологічних наук Registration Date 11-01-2024 Organization Taras Shevchenko National University of Kyiv popup.description2 Research object: immunomodulatory properties of bacteriophage–derived doublestranded RNA (Larifan). Purpose of work: to investigate the effect of Larifan on the metabolic profile of isolated alveolar macrophages in healthy C57BL/6 mice of different sexes and ages and monocytes from healthy male donors. Research methods: isolation of murine alveolar macrophages, hydrodynamic, cultural (short-term cultivation of murine alveolar macrophages and human peripheral blood monocytes), colorimetric (determination of arginase activity of monocytes, Griess reaction), flow cytometry (determination of phagocytic activity, oxidative metabolism and expression of phenotypic markers by phagocytes). Results and novelty: The bacteriophage–derived double-stranded RNA (Larifan) has been shown for the first time to induce anti-inflammatory polarized activation of resting alveolar macrophages in C57Bl/6 mice, which are distinguished by the predominance of immune reactions of the M1 (Th1) profile and cell-mediated anti-infective immune response, as well as a reduced number of Foxp3+ Treg, in vitro. With this study, we have also demonstrated for the first time unique metabolic profile of resting peripheral blood monocytes from healthy young male exposed to Larifan in vitro, which is favorable for their antiviral activity. In the course of the study, new data were obtained about the dependence of the Larifan impact on murine phagocyte metabolic profile on gender and age. Phagocytes from aged C57Bl/6 mice were more responsive to the preparation as compared to their young counterparts. Most pronounced anti-inflammatory metabolic skew was registered in alveolar resident macrophages from old male mice. Product Description popup.authors Dovhyi Roman S. Rudyk Mariia P. Serhiichuk Tetiana M Yuliia M. Yumyna popup.nrat_date 2024-01-11 Close