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Information × Registration Number 0224U000868, 0122U002425 , R & D reports Title Identification of the Brassica rapa tubulin gene family and characterization of their genomic organization in different subspecies popup.stage_title Head Pirko Yaroslav V., Доктор біологічних наук Registration Date 14-01-2024 Organization Department for special training National academy of science of Ukraine at Kiev University popup.description2  During the second work stage, the variability of the tubulin gene intron sequences for various subspecies of B. rapa was analyzed, and the expected β- and γ-tubulin introns amplicon lengths were established. We genotyped samples of various B. rapa subspecies from the existing collection using the TBP method, based on β-tubulin intron length polymorphism. The obtained genotype data are comparable to the expected lengths of the target amplicons of β-tubulin introns. Based on these data, phylogenetic relationships between the analyzed subspecies and genotypes were reconstructed. Genotyping based on the γ-tubulin intron length polymorphism was carried out, the resulting amplicon profiles of the of β- and γ-tubulin introns were analyzed for the presence of typical amplicon lengths specific to each of the B. rapa subspecies. The obtained results made it possible to develop DNA barcoding approaches at the molecular genetic level. Product Description popup.authors Afanasyeva Kateryna Sergiyivna Blume Rostislav Ya. Pirko Yaroslav Vasylʹovych Rabokon Anastasiia M. Sakharova Vladislava Sviridova Katerina O. Chopei Mariana Ihorivna popup.nrat_date 2024-01-14 Close