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Information × Registration Number 0224U001090, 0122U001660 , R & D reports Title Development of nanoscale liposomal delivery systems for biologically active substances and study of their antitumor activity popup.stage_title Head Berest Volodymyr P., Доктор фізико-математичних наук Registration Date 17-01-2024 Organization V.N. Karazin Kharkiv National University popup.description2  The object of study is the effect of liposomes of different lipid compositions containing GS on the viability, proliferative, and migratory potential of L929 cells. The work aims to create liposomes loaded with gramicidin S and study their bioavailability, toxicity, lytic action, and antiproliferative and antimigratory effects. Methods of study: fluorescence microscopy, confocal laser, and optical microscopy. Nano-sized, fluorescently labeled liposomes of a defined lipid composition with different surface charge densities and membrane microviscosity were created to encapsulate the antimicrobial peptide gramicidin S (GS). The influence of different concentrations of GS, empty liposomes, and liposomes loaded with GS, on the viability, adhesive, proliferative and migratory properties of fibroblasts in planar and organoid-like cell cultures was studied. Gramicidin S slows down the repair of the monolayer defect due to a decrease in the migratory activity of L 929 fibroblasts and suppression of their adhesive properties. In 3D culture, fibroblasts show greater resistance to the damaging effects of GS compared to cells in a monolayer. Empty liposomes do not affect the morphology and function of cell culture. Liposomal peptide delivery leads to a 1.5-fold increase in the effective concentration of GS capable of influencing the viability and adhesive properties of cells in a monolayer. Liposomes loaded with GS in concentrations of 10, 25, 50, 75 μg/ml inhibit the ability of spheroids to adhere and the ability of fibroblasts to migrate and proliferate. Liposomes with a negative surface charge containing cholesterol better deliver the drug. The possibility of using an antimicrobial drug for anticancer therapy has been shown for the first time. Optimization of the antimicrobial and antineoplastic therapy by using nano-sized lipid vesicles is proposed, and the prospects of creating new encapsulated forms of GS for targeted delivery are determined. Product Description popup.authors Bozhok Galyna A. Zabelina Irina A. Kuzeva Olga V. Sichevskа Larysa V. Chepeleva Liudmila V. popup.nrat_date 2024-01-17 Close