Search academic texts

Information × Registration Number 0224U001380, 0121U108467 , R & D reports Title To improve the system of diagnostics and to develop modern means of specific prevention of anaerobic infections of animals popup.stage_title Head Zhovnir Oleksandr M., Registration Date 22-01-2024 Organization Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine popup.description2  Primers were developed based on the sequence of the csa gene of the reference strain C. septicum; a PCR test system was created for the detection of RNA of pathogenic clostridia by 45-cycle amplification with an annealing temperature of 60 C and the content of magnesium ions in the reaction mixture at the level of 2.2 mM/?L; The overall specificity of the PCR-RT test for the C. septicum target gene was 100%, and the sensitivity for samples with 100 genome copies or more was 100% (Wilson's 95% confidence interval: 99.3 to 99.9%). The agreement threshold of OD >+0.3 obtained as a result of research is a sufficient indicator for reliable detection of amounts of the pathogen in the threshold value of 100 or more copies of the genome in the reaction. Monitoring studies were conducted and the etiological structure of anaerobic infections of animals in the territory of Ukraine was studied. More than 1.5 mln. was received from the implementation of scientific developments of the laboratory. UAH special funds. Product Description popup.authors popup.nrat_date 2024-01-22 Close