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Information × Registration Number 0224U001381, 0123U100538 , R & D reports Title Створення нових підходів для діагностики SARS-CoV-2 popup.stage_title Head Tarasov Oleksandr A., Registration Date 22-01-2024 Organization Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine popup.description2  Methodological recommendations for the diagnosis of SARS-CoV-2 using looped isothermal amplification (LAMP) have been developed. A set of primers was developed and the conditions for loop isothermal amplification (LAMP) were optimized for the rapid and efficient diagnosis of SARS-CoV-2 virus by real-time RT-LAMP and RT-LAMP with colorimetric evaluation of the reaction result by the endpoint (color change of the reaction mixture). whole-genome sequencing of 8 isolates of SARS-CoV-2 virus was performed and among the sequenced isolates five sublines of the Delta variant were detected: two AY.33 (B.1.617.2.33), one AY.36 (B.1.617.2.36), one AY.122 (B.1.617.2.122), one B.1.617.2 and two AY.4.2 (B.1.617.2.4.2). One of the eight isolates was assigned to the Alpha variant, which is a representative of the B.1.1.7 lineage. Six sequences have been deposited in the database https://gisaid.org, accession number EPI_SET_230516yp (doi:10.55876/gis8.230516yp) Product Description popup.authors popup.nrat_date 2024-01-22 Close