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Information × Registration Number 0224U001434, 0121U107835 , R & D reports Title The comprehensive substantiation of ecological and genetic bases of terroir identification in Ukraine popup.stage_title Head Vlasov Viacheslav V., Доктор сільськогосподарських наук Registration Date 23-01-2024 Organization National Research Center "VE Tairov Institute of Viticulture and Enology" popup.description2  The report on the GDR is laid out: on 23 pages, 4 tables, 3 figures, 29 literary sources. Keywords: wine yeast strains, grape berry microbiome, sequencing, ribosomal RNA, nucleotide sequence analysis, Sukholimansky white, phylogenetic analysis programs The object of research is Saccharomycescerevisiae yeast strains. The subject of research is the nucleotide sequences of loci and loci - ITS1_5.8S_ITS2 The purpose of the work is to conduct a phylogenetic analysis of yeast strains isolated from the Sukholimansky white variety. Research methods - analysis using the MEGA nucleotide sequence analysis program. Research results. The analysis of the open database of the foreign collection (Japan) was made, and the nucleotide sequences of 8 yeast strains isolated at the National Research Center "IViV named after V.E. Tairov" from the Sukholimansky white variety and deposited in the specified collection. The identification of the species of yeast strains isolated on the Sukholimansky white variety was carried out, it was determined that 8 studied strains belong to the Sacharomycescerevisiae species The capabilities of programs for analyzing nucleotide sequences were evaluated for phylogenetic studies, and the version of the MEGA program was chosen for further work. An analysis of the nucleotide sequences of ribosomal RNA genes of wine yeast strains isolated from the Sukholimanskyi white variety in different regions of the Odesa region was carried out, which showed the presence of clustering of groups of the studied strains (the first cluster is strains isolated from the vineyards of the Tairovske State Enterprise, the second is strains isolated from vineyards of the O.V. Suvorov SE DG"), thereby demonstrating the influence of location and environmental conditions on the diversity of wine yeast strains. Product Description popup.authors Buzovska Marina B Bulaieva Iuliia Yu. Vozniuk Nadiia Volodymyrivna Liashenko Halyna V. Melnyk Ella B Mulyukina Nina Anatoliyivna Popova Anna K Suzdalova Vira I popup.nrat_date 2024-01-23 Close