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Information × Registration Number 0224U001553, (0122U000664) , R & D reports Title Molecular and cellular bases of development of socially significant human diseases and creation on this basis of modern nanobiotechnologies for prevention, diagnosis and treatment popup.stage_title Розробка електрохімічних перетворювачів для створення кондуктометричних та потенціометричних біосенсорів. Розробка умов для вирощування культури тканин раувольфії зміїної. Проведення клініко-геніалогічного аналізу серед хворих з ССТ Head Telehieiev Henadii D., Доктор біологічних наук Registration Date 25-01-2024 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 The aim of the work is to study the molecular and cellular bases of the development of socially significant human diseases and to create on this basis modern nanobiotechnologies for prevention, diagnosis and treatment. Development of approaches to the creation of electrochemical biosensors for direct detection of substrates and inhibitory analysis of toxic substances in environmental monitoring. Development of conditions for long-term cultivation of Rauwolfia snake tissue cultures, development of methods for isolation of target and major alkaloids. Analysis of the association of polymorphic gene variants (ZNF469, ACE1) in a group of patients with cases of keratoconus of varying severity. Analysis of the expression of key enzymes of redox metabolism (REDOX) in tumor cells, which is sensitive to chemoradiotherapy. popup.description2 Enzyme biosensors for direct analysis of substrates and inhibitory determination of toxic substances of various origins were developed, and their laboratory prototypes were made. Biosensors were created based on the effect of enzyme inhibition and the measurement of responses related to enzymatic activity in the absence and presence of an inhibitor, followed by their quantitative determination. The developed biosensor systems are adapted to large-scale production. The protective effect of vitamin E against cancer-associated oxidative stress in DU145 prostate cancer cells was demonstrated through vitamin E-dependent specific inhibition of TXN2 and GPX4 gene expression levels. The modulator effect of vitamin E on the activation of CDH1 expression and the repression of the EMT-inducer SNAIL transcription factor was shown, indicating vitamin E-dependent inhibition of the EMT phenotype of DU145 prostate cancer cells and its resistant culture DU145 under exposure to vitamin E. A group of 155 patients with keratoconus Genotyping was carried out by the method of allele-specific PCR. The results of genotyping of polymorphic variants of the rs9938149 ZNF469 gene were confirmed by Sanger sequencing. Based on the rs9938149 C/A analysis of the ZNF469 gene polymorphism, it is shown that the homozygous SS genotype can be considered protective for a more aggressive course of the disease, and rs9938149 can be used as an informative genetic marker for the prognosis of keratoconus. The K-27M strain of R. serpentina was grown on the classic MS medium and the 10C medium specially developed for tissue culture of this species, which differed in mineral base, vitamin and sugar content. It was established that the content of dry biomass in the raw tissue of R. serpentina when grown on 10С medium was 7.6%, which is two times more compared to cultivation on MC medium. The accumulation of indole alkaloids in the dry biomass of tissue culture of the K-27M strain of R. serpentina on both nutrien Product Description popup.authors Arkhypova Valentyna M. Tetiana A. Serheieva Ihor O. Andrieiev Svitlana V. Marchenko Oleksandra V. Horodna Liudmyla A. Livshyts Yurii V. Karpenko Mykhaylo V. Dybkov Yaroslav I. Korpan Viktor A. Kunakh Oleksii P. Soldatkin Olha A. Biloivan Iryna I. Konvaliuk Liudmyla P. Shvachko Yarmoliuk Serhii M. Oleksandr O. Soldatkin Shkotova Liudmyla V Yarynka Daria V Dziadevych Serhii V. popup.nrat_date 2024-01-25 Close