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Information × Registration Number 0224U002111, 0123U101083 , R & D reports Title Cellular and humoral mechanisms of neuroprotection in in vivo and in vitro models of brain damages popup.stage_title Head Skybo Halyna H., Доктор медичних наук Registration Date 06-02-2024 Organization Bogomolets Institute of Physiology of the National Academy of Sciences of Ukraine popup.description2  The conditions for isolation and cultivation of neuronal cells from the brain and enteric nervous system of rat intestines were optimized. A morphological analysis was carried out to reveal specific cell markers for identification and assessment of the state of neurons and neuroglia in cultures. Cultivation of Lactobacillus rhamnosus and Lactococus lactis strains was optimized in order to develop the products of their metabolism under standardized conditions for further use in experiments. Expression of interleukin 1β (IL-1β), marker of glutamatergic synapses PSD-95 and brain-derived neurotrophic factor (BDNF), involved in the processes of inflammation and synaptic plasticity, was estimated in middle-aged mice hippocampus under conditions of systemic metabolic disorders (mouse line ApoE-/ -) and various dietary influences. It was investigated the localization and integration of GFP/DAB-positive oligodendrocytes, generated from human neuroepithelial stem cells and grafted into the somatosensory cortex of the rat brain after ischemic injury simulation. Technique to study spatial distribution of cells has been developed. The technique is implemented in a software written in Object Pascal. It is used in the context of cluster analysis, when the criterion for belonging to a spatial cluster is the threshold value of the edge length of the Delaunay graph. Product Description popup.authors Kovalenko Tetyana М. Lushnikovа Irina V. Nikonenko Olexander G. Osadchenko Iryna O. Patseva Maryna A. Skybo Galyna G. Smozhanik Kateryna G. Tsupykov Oleg M. Shepilov Dmytro R. popup.nrat_date 2024-02-06 Close