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Information × Registration Number 0224U002550, 0123U103005 , R & D reports Title Cell-biological and molecular-genetic mechanisms of salt and drought tolerance of barley and prospects of their regulation for improvement of cereal crops popup.stage_title Head Pirko Yaroslav V., Доктор біологічних наук Registration Date 18-02-2024 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2  Amplification of the coding sequence of the SCOR potassium ion channel gene of Hordeum marinum was carried out. The sequence was cloned into pDonor 221 and pYES-DEST52 vectors for further sequencing. The obtained CDS H.mSCOR plasmid clones are planned to be used for the creation of genetic constructs and further transformation of plants or heterologous systems in order to clarify the functional characteristics and physiological functions of this channel and in the creation of salt-resistant lines. As a result of CDS sequencing of clones, the CDS sequence of H.mSCOR with a length of 1976 bp was obtained. verified CDS sequence of H.mSCOR was used for comparative analysis with CDS sequences of SCOR genes of other plant species. It was determined that the isolated gene has significant differences with its domesticated relative H. vulgare. The gene structure of the autophagosome-forming proteins ATG2, ATG7, ATG8 of H.vulgare and H.marinum was analyzed. Potential markers of intronic polymorphism (ILP-markers) were created for introns characterized by the largest interspecies length polymorphism. The created ILP-markers can be used in further studies of autophagy regulation processes under salt stress. Product Description popup.authors Yemets Аllа І. Blume Rostislav Ya. Borova Mariya M. Buziashvili Anastasiia Yu. HORDYNSKYI SERHII O. Danylenko Ihor A. Demkovych Andrii Ye. Kvasko Anna Yu. Kyrylenko Viktor M. Koval Olimpiada P. Novozhylov Dmytro O. Yaroslav V. Pirko Plokhovska Svitlana H. Pryvalikhin Serhii M. Pushkarova Nadiia O. Rabokon Anastasiia M. Shysha Olena M. popup.nrat_date 2024-02-18 Close