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Information × Registration Number 0224U002826, 0121U113399 , R & D reports Title Novel composites based on cerium oxide nanoparticles and carbon enterosorbents for acute radiation sickness therapy popup.stage_title Head Nikolaiev Volodymyr Н., Доктор медичних наук Registration Date 05-03-2024 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2 CeO2 nanoparticles of 14nm and 4.2nm did not show cytotoxic effects on all types of studied cell lines, increased the proliferative activity of CMM, CFTE-29 and HaCaT, caused a significant increase in protein and DNA biosynthesis in MDBK, CMC, MAM, and MCA cell lines. Visual analysis showed the accumulation of CeO2 nanoparticles in the cytoplasm and on the membrane surface of all studied cells. The antioxidant activity of CeO2 nanoparticles of 14nm and 4.2nm in size was experimentally demonstrated, which demonstrated the protection of KMM and MDBK cell lines from the cytotoxic action of H2O2, as well as the reduction of total reactive oxygen species and their biochemically unchanged action. The radioprotective effect of CeO2 nanoparticles of 14nm and 4.2nm on KKM and MAEK cells in the conditions of their preliminary incubation for 24 or 48 hours and subsequent ɣ-irradiation at a dose of 18 and 24 Gy was established. It was found that the radioprotective efficiency of CeO2 nanoparticles with a size of 4.2nm relative to CCM cells was slightly higher than 14nm, and with increasing dose of ionizing radiation, the radioprotective effect of CeO2 nanoparticles becomes more pronounced. It was shown that intraperitoneal administration of a suspension of CeO2 nanoparticles at a daily dose of 10 mg/kg for 2 weeks after irradiation at a dose of 6 Gy Wistar rats caused an increase in antioxidant potential of irradiated animals, had positive effect on blood cells, oxidative stress indices and morphological structure of the liver and kidneys. A scale of biomarkers for the analysis of the adsorption potential of carbon enterosorbents has been developed. The test scale includes three groups of markers: 1.Multi-charged dyes; 2.Bioactive substances, 3.Medical substances and xenobiotics. Samples of highly activated carbon adsorbents from different types of natural and synthetic raw materials were obtained and the technology of obtaining oral dispersed forms enterosorbents was developed Product Description popup.authors Nikolaiev Volodymyr Н. Sarnatskaya Veronika V. popup.nrat_date 2024-03-05 Close