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Information × Registration Number 0224U032160, 0123U100462 , R & D reports Title Study of vital activity peculiarities of biotechnological plants after genomic modifications popup.stage_title Head Morhun Bohdan V., Доктор біологічних наук Registration Date 31-07-2024 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description2 The following work was carried out: genetic constructs containing the T-DNA gene of phosphite oxidoreductase ptxD from Pseudomonas stutzeri strain WM88 under different regulatory sequences were created; it was found that transgenic Nicotiana tabacum plants of the Petit Navana variety do not have the ability to assimilate phosphite as the only source of phosphorus. For the first time, the possibility of obtaining transgenic maize lines of Ukrainian selection by Agrobacterium-mediated genetic transformation in vitro with stable inheritance of transgenes was proved. As a result of inter-factorial analysis (genotype, composition of cultivation media), it was found that among the eight tested Triticum spelt breeding lines, genotypes 3863, 3865 and 3995 have the highest frequency of regeneration, rooting and survival to septic conditions and are promising for obtaining biotechnological plants. The optimal conditions for cytogenetic studies of durum wheat roots were selected. When co-cultivating colonies of ascending yeast strains and their petite mutants, the reaction of parental strains to the neighborhood of their petite mutants was revealed - gradual absorption of the petite mutant colony. Product Description popup.authors Bannykova Mariia О. Nitovska Iryna O. Pronina Olha V. popup.nrat_date 2024-07-31 Close