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Information × Registration Number 0224U032163, 0120U100849 , R & D reports Title Targeted genome changes and pleiotropic effects in genetically transformed plant systems popup.stage_title Head Kuchuk Mykola V., Доктор біологічних наук Registration Date 01-08-2024 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description2  During the reporting period, transgenic plants and transgenic roots of several plant species were obtained. Intertribal highly asymmetric somatic hybrids were also obtained, which will be used in further studies of the inheritance of transgenic traits and possible pleiotropic effects in the expression of heterologous genes. A platform and genetic vectors were also developed for targeted editing of the rapeseed genome using the CRISPR/Cas9 system in order to change the fatty acid composition of B. napus L. rapeseed and increase the accumulation of oleic acid. Research work on the project was carried out using modern methods of in vitro culture, genetic transformation and genome editing, the Golden Gate method of modular molecular cloning, molecular biological analysis and bioinformatics. The obtained results correspond to high international standards. Product Description popup.authors Bulko Olha V. Vasylenko Maksym Yu. Duplii Volodymyr P. Kyrpa Tetiana M. Loshyna Liudmyla H. Matvieieva Nadiia A. Ovcharenko Olha O. Potrokhov Andrii O. Ratushniak Yakiv I. Volodymyr A. Rudas Sindarovska Yana R. Symonenko Yurii V. Shakhovskyi Anatoli M. Shcherbak Nataliіa L. Yaroshko Olha M. popup.nrat_date 2024-08-01 Close