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Registration Number

0224U032360, 0123U101139 , R & D reports

Title

Study of the regenerative potential of cell therapy in orthopedics and traumatology

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Лабораторні дослідження клітинних культур

Head

Holovakha Maksym Leonidovych, д.мед.н.

Registration Date

22-09-2024

Organization

Zaporizhzhya State Medical University

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Object of study - mesenchymal cells of the stromal-vascular fraction. Purpose - to study and substantiate the use of autologous cell cultures obtained from the stromal-vascular fraction for the treatment and recovery of traumatological and orthopedic patients. Research methods - morphometric, biochemical, histochemical, histological, clinical, radiological, statistical. Results of the research and their novelty. For the first time, the composition and number of stromal mesenchymal cells were compared by immunohistochemical method in the stromal-vascular fraction obtained from the infrapatellar fat body and subcutaneous tissue. The morphological features and differences between the SVF of Goff's body and subcutaneous fat were investigated, and their potential for use was determined. Immunohistochemical study of the stromal-vascular fraction of the Goff's fat pad, subcutaneous adipose tissue, biopsy material of the Goff's pad and subcutaneous adipose tissue using monoclonal antibodies revealed that in the SVF of the Goff's fat pad and subcutaneous adipose tissue, the relative number of cells with the immunohistochemical profile CD105+ CD73+ CD34+ CD31- CD45- in the standard field of view (x200) of the SVF of the Goff's fat pad was 2.89 (1.57; 4.00) %, whereas the SVF of subcutaneous adipose tissue was 6.15 (5.38; 10.71) %, which is 2.13 times higher, but this difference is not statistically significant (p > 0.05). In the biopsy material of the Hoffa's fatty body, the relative number of cells with the immunohistochemical profile CD105+ CD73+ CD34+ CD31- CD45- in the standard field of view was 7.72 (6.59; 9.43) %, in the biopsy material of subcutaneous adipose tissue - 11.11 (8.46; 13.45) %, which is 1.44 times more (p > 0.05).

Product Description

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Avramenko Yuliia M.
Bukhtiiarova Nina V.
Danukalo Maksym V.
Maslennikov Serhii O.

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2024-10-01