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0224U032684,
(0123U102869)
,
Development an aerosol liposomal form of curcuminoids and miRNAs complex for the treatment of Alzheimer's disease earlystage
Визначення дозозалежної ефективністі аерозолю ліпосомальної форми комплексу з вмістом куркуміну і miR-101 за однократного застосування
Shulha Serhii M., Доктор біологічних наук
01-12-2024
Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine
Development of a new drug – aerosol lipid target systems containing curcumin and microRNA (drug) for the treatment of neurodegenerative diseases. Determination of anti-inflammatory and anti-amyloid activity of the drug, its effectiveness in relation to the dose and duration of action and aftereffects in vivo
A liposomal emulsion aerosol was obtained by hydration of lipid films followed by sonication and extrusion through membranes with pore openings of 200 nm, 100 nm, and 50 nm in steps. It has been shown that curcumin is incorporated into the lipid bilayer and miR-101 into the liposome aqueous core. It was found that the spray angle and aerosol deposition profiles in the nasal passages depend on the concentration of the respective active pharmaceutical ingredients and the state of the nasal epithelium. Curcumin and microRNA were detected in rat brain tissue samples 40 min after administration. Two experimental models of Alzheimer's disease in vitro were created by incubating blood mononuclear cells (hPBMCs) in suspension and in immobilized state with β-amyloid peptide aggregates. The expediency of using models based on cells in suspension and in the immobilized state for further research has been proved. An experimental model of the early pre-symptomatic stage of Alzheimer's disease in old (14 months old) rats was created by intrahippocampal single injection of β-amyloid peptide aggregates_Human 42. The dynamics of neuroinflammation development in the hippocampus and neocortex of the brain of animals with Alzheimer's disease model is shown... The dynamics of neuroinflammation development in the hippocampus and neocortex of the brain of animals with Alzheimer's disease model is shown. In an experimental model of Alzheimer's disease in rats, the dynamics of the level of biomarkers of inflammation, oxidative stress and amyloidogenesis was first established by the effective concentration of curcumin and miR-101 in liposomes after their nasal administration, namely 0.55 mM Cur + 1.25 nM miR-101. It was found that the effective duration of the course of aerosol application of the liposomal form of the complex containing curcumin and miR-101 in animals with an Alzheimer's disease model should be at least 15 days.
Andriіash Hanna S.
Bandura Uliana H.
Berchenko Olha H.
Blume Yaroslav B.
Borova Mariia M.
Kot Kateryna V.
Linskyi Ihor V.
Plokhovska Svitlana H.
Sokolik Viktoriia V.
Tihunova Оlena O.
2024-12-01
Updated: 2025-12-06
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