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Registration Number

0224U032697, (0124U003831) , R & D reports

Title

Development of a broad-spectrum antimicrobial agent based on the extract of the leguminous plant Indigofera spicata

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Отримання екстрактів I. spicata та перевірка їх антимікробної та протигрибкової активності

Head

Stasyk Oleh V., Доктор біологічних наук

Registration Date

02-12-2024

Organization

Institute of Cell Biology of the National Academy of Sciences of Ukraine

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The goal is to create a prototype of a new broad-spectrum antimicrobial agent for local disinfection of wounds or other skin damage based on the extract of the leguminous plant Indigofera spicata, which contains unique amino acid analogs that are cytotoxic for microorganisms, as well as to test its effectiveness on cell models in vitro and in laboratory animals vivo.

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During the implementation of the intermediate stage of the project, the endemic Australian plant Indigofera spicata was acclimatized for the first time, which made it possible to grow it in Ukraine under the conditions of a greenhouse and free soil. It was shown that the content of key compounds (canavanine, indospicine) in the green biomass of acclimatized plants did not differ significantly from plants growing in Australia, which indicates the possibility of using acclimatized plants as a cheap and accessible source of arginine analogs. Water-ethanol and methanol-chloromethane extracts were obtained from seeds, green biomass, and roots of Indigofera spicata. The extracts were characterized by the content of arginine and its analogs. Indigofera spicata extracts treated with recombinant human arginase I were also obtained to test the hypothesis of higher antimicrobial efficacy of arginine-free extracts. Growth kinetics of model strains of non-pathogenic microorganisms (E. coli, B. subtilis, P. aerurinosa, S. cerevisiae, C. phaffii, C. albicans) were determined. It was established that the antimicrobial activity of extracts and individual arginine analogs was present only in arginine-free conditions.

Product Description

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Vovk Olena I.
Demash Dmytro V.
Polishchuk Nikita V.
Shuvaieva Halyna Yu.

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2024-12-02