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Information × Registration Number 0224U032708, (0123U102740) , R & D reports Title Silvicultural and molecular genetic principles of increasing the biotic resilience of Scots pine stands in the context of adaptation to climate change and anthropogenic pressure popup.stage_title Лісівничі та молекулярно-генетичні засади підвищення біотичної стійкості деревостанів сосни звичайної в контексті адаптації до змін клімату та антропогенного навантаження Head Kovalova Valentyna A., Кандидат біологічних наук Registration Date 02-12-2024 Organization Ukrainian national forestry university popup.description1 To develop scientific and methodological bases of biotechnological applications aimed at growing biologically stable pine forests in Ukraine under new ecological conditions by introducing close-to-nature forestry, to determine the role of defensin genes in the resistance of pine to complex Ips acuminatus-Ophiostoma for further application in breeding programs, as well as to investigate the adaptogenic properties of bacteria for increasing the resistance of pine seedlings to stress popup.description2  The research was conducted in pure and mixed forest stands of artificial and natural regeneration across 18 trial plots. The distribution of pine stands by forest types, age, origin, spatial structure, and taxonomic indicators (composition, height, diameter, completeness, crown closure, annual growth, productivity) was analyzed. Additionally, fallout, litter, dielectric properties (impedance, polarization capacity), and the species composition of phytopathogens and entomophytes were characterized. High vitality and biotic resistance of pine stands were identified. The study examined the influence of phytopathogenic fungi Ophiostoma sp. and Fusarium verticillioides on the expression of defensin genes in Scots pine. It was found that inoculation of seedlings with F. verticillioides significantly increased the expression levels of these genes. Treatment with endophytic Pseudomonas bacteria enhanced the immune response of the plants to phytopathogenic fungi. Abiotic stressors associated with global climate change also modulated the expression of defensin genes. The differential expression patterns confirmed their usefulness as markers for selecting pine genotypes resistant to multiple stressors. The application of Pseudomonas bacteria improved pine seed germination, stimulated root system growth, and increased seedling biomass. An increase in chlorophyll content, respiration intensity, and transpiration rates was observed in bacterized seedlings. These findings highlight the potential of endophytic bacteria as bioagents for protecting pine seedlings from diseases and enhancing their resilience to adverse environmental conditions. The results provide a basis for developing innovative methods to protect forest crops. Product Description popup.authors Kit Oleh Yu. Korol Mykola M. Kramarets Volodymyr O. Krynytska Olha H. Krynytskii Hryhorii T. Lavnyi Vasyl V. Maguran Volodymyr K. Shalovylo Yuliia I. Yusypovych Yurii M. popup.nrat_date 2024-12-02 Close