Search academic texts

Information × Registration Number 0224U032715, (0123U104868) , R & D reports Title Effective treatment of peripheral nerve injury by biomimetic 3D scaffolds popup.stage_title Оптимізація мікроархітектури скаффолда та оцінка ефективності поєднання матеріалу GelMA з дизайном оптимальної мікроархітектури скаффолда для регенерації сідничного нерва при терапії ТПН Head Nana V. Voitenko, Доктор біологічних наук Registration Date 02-12-2024 Organization Private Institution Dobrobut Academy popup.description1 The aim of this project is to determine the effectiveness of novel types of scaffolds for treatment of peripheral nerve injuries. popup.description2 Peripheral nerve injuries remain a significant challenge for medicine due to the limited possibilities of achieving full functional recovery. This project has developed a novel approach to treating such injuries, utilizing advanced 3D printing technologies and biocompatible materials. A 3D-printed microchannel scaffold was created, mimicking the natural structure of nerves and enabling directed nerve fiber growth. It has been established that the use of such an implant significantly enhances functional recovery compared to other methods. In a model of sciatic nerve injury in experimental animals, progressive improvement in functional recovery indicators was observed over six months post-implantation. Research involving silicone tubes, which served as temporary conduits for regeneration, demonstrated the limited efficacy of this approach. It was shown that functional recovery in such cases stabilizes at early stages and does not progress over time. In contrast, the 3D-printed microchannel implant continued to support nerve fiber regeneration even at later observation stages. The group using the 3D implant exhibited more harmonious axon growth, demonstrating the effectiveness of this approach. To analyze regeneration mechanisms, viral vectors containing green fluorescent protein (GFP) were employed. This method confirmed successful cell transduction in the proximal section of the injured nerve and allowed the assessment of cellular condition and morphology during the recovery process. The dynamics of functional recovery were evaluated using the sciatic functional index (SFI). It was demonstrated that the best outcomes are achieved with the 3D microchannel scaffold, while other methods, such as neurorrhaphy and the use of empty tubes, showed significantly lower efficacy. The developed technologies have substantial potential to improve Ukraine's healthcare system. Product Description popup.authors popup.nrat_date 2024-12-02 Close