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Information × Registration Number 0224U032753, (0123U102613) , R & D reports Title Cumulative neurotoxic effect of multicomponent pollution by airborne particles and neuroactive pharmaceuticals, biomaterials (including SARS-CoV-2), toxic metals and its prevention popup.stage_title Здатність біоматеріалів, в тому числі спайкового білку SARS-CoV-2 та фізіологічних білків, до утворення біокорони на поверхні природних/синтезованих РМ. Аналіз спонтанного та стимульованого утворення АФК у нервових терміналях після внесення прир./синт. зразків РМ та комплексів PM і забруднювачів. Експерименти in vitro щодо комбін. впливу зразків комплексів РМ і важких металів на життєздатність клітин. Синтез сорбентів для важких металів шляхом гідроксилювання графен. і кремн. наночастинок. Комбін. вплив прир. та синт. зразків РМ та нейроактивних фарм. забруднювачів на ключові характеристики глутамат- та ГАМКергічної нейротрансмісії у нервових терміналях та спонтанну і стимульовану генерацію АФК. Експерименти in vitro щодо комбін. впливу зразків комплексів прир./синт. РМ і нейроактивних фарм. забруднювачів на життєздатність клітин. Синтез сорбентів для нейроактивних фарм. препаратів шляхом функціоналізації графен. і кремн. наночастинок кислотними і основними функціональними групами. Head Tetiana O. Borisova, д.б.н. Registration Date 03-12-2024 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description1 The aims are investigation of cumulative neurotoxic effects of multicomponent pollution by airborne PM and neuroactive pharmaceuticals, biomaterials and toxic metals using own approach on assessment of acute neurotoxicity using brain nerve terminals, in vitro and in vivo animal models; identification of types of PM that can be carriers for these pollutants; molecular mechanisms of neurotoxicity; interconnection between PM and susceptibility to SARS-CoV-2 infection; and countermeasures. popup.description2 For the first time, the diversity of the interference effects of neuroactive pollutants, namely heavy metals, pharmacological drugs, and carbon-containing smoke particles, regarding the impact on key presynaptic processes was revealed. It was proved for the first time that the physiological proteins, albumin and mucin, were capable of partially mitigating the disturbance of the extracellular level of L-[14C]glutamate and [3H]GABA, as well as the membrane potential in rat brain nerve terminals, caused by carbon-containing smoke particles from the combustion of wood and plastic. It was shown for the first time that the combined application of Cd2+ and Pb2+ and carbon-containing smoke particles from wood and medical masks reduced Cd2+ and Pb2+ - stimulated ROS generation in rat brain nerve terminals. The neurological hazard from multi-pollution with cadmium and lead on the one hand and smoke particles from wood and medical masks on the other hand due to a violation of the redox balance in rat brain nerve terminals was proven for the first time. For the first time, it was proved that various neuroactive drugs, potential environmental pollutants, did not change (pregabalin, levetiracetam), masked (oligomycin, rotenone) or enhanced (remdesivir, cinazepam, amphotericin) the neurotoxic effects of carbon-containing smoke particles from wood and medical masks in rat brain nerve terminals. The obtained experimental data on the neurological impact from multi-pollution with cadmium and lead and carbon-containing smoke particles due to the violation of the redox balance, as well as the data on the cumulative neurotoxic effect of the complexes of carbon-containing smoke particles and remdesivir, cinazepam and amphotericin can be used to improve the methodology of health risk assessment during environmental monitoring.   Product Description popup.authors Dudarenko Maryna V. Panas Ihor D. Pastukhov Artem O. popup.nrat_date 2024-12-03 Close