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Information × Registration Number 0224U032778, (0123U103497) , R & D reports Title Anti-inflammatory and wound-healing agent containing the composition of the cannabimimetic N-stearoylethanolamine with chitosan hydrogel popup.stage_title Оцінювання біотолерантності та ранозагоювальних властивостей створеної композиції in vitro та in vivo Head Stoika Rostyslav S., Доктор біологічних наук Registration Date 04-12-2024 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description1 The project aims to develop a new medicinal product, which is a composition of the cannabimimetic N-stearoyl ethanolamine (NSE) in a chitosan hydrogel, for healing superficial wounds and suppressing inflammation in laboratory animals. popup.description2  Chitosan, derived from the abundant natural biopolymer chitin, is known for its hemostatic and wound-healing properties. It is a versatile platform for delivering drugs, nucleic acids, and other biologically active compounds. Within this project, two multifunctional preparations were developed to promote the healing of acute skin wounds: chitosan-based hydrogels in the form of films and chitosan-based ointments. The hydrogel formulation incorporates hyaluronic acid as a stimulator of regenerative processes, while the ointment formulation includes linseed oil, ampicillin, polyvinylpyrrolidone, and 1,2-propylene glycol. Both preparations are enriched with the cannabimimetic compound N-stearoylethanolamine (NSE), recognized for its anti-inflammatory properties. We confirmed the biodegradability of these preparations and the biocompatibility of their components. Scanning electron microscopy revealed that the preparations exhibit a coarse-porous structure, facilitating their adherence to wound surfaces. Experimental studies on wound treatment in laboratory mice demonstrated the robust wound-healing efficacy of these formulations, evidenced by accelerated wound closure, shortened epithelialization time, and enhanced remodeling of collagen fibers. Topical application of the NSE-containing ointment reduced wound-associated inflammatory responses, as reflected in time-dependent changes in specific immune cell profiles. Notably, no significant alterations were observed in the tumor necrosis factor-alpha (TNF-α) levels and interleukin-6 (IL-6) in plasma samples from the treated mice, suggesting limited systemic inflammatory effects. Thus, we have developed user-friendly, domestically produced products for effective wound healing. Due to their amphiphilic nature, these preparations can be further functionalized with various biologically active substances, including poorly water-soluble compounds, antibiotics, and antioxidants. Product Description popup.authors Ivasechko Iryna I. Antoniuk Volodymyr O. Hula Nadiia M. Dumych Tetiana I. Lutsyk Maksym D. Mehed Oksana F. Panchuk Rostyslav R. Skorokhid Nadiia R. Stoіka Rostyslav S. popup.nrat_date 2024-12-04 Close