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Information × Registration Number 0224U032833, (0124U002546) , R & D reports Title Bacteriophages as biological agents for the control of bacterial complications in victims of gunshot and mine-explosive injuries popup.stage_title Виділення і характеристика бактеріофагів до ідентифікованих патогенних мікроорганізмів, та оцінка перспективності їх окремого чи комбінованого використання для терапії гнійних бактеріальних посттравматичних ускладнень і профілактики їх розвитку. Head Shevchenko Oleksii V., Кандидат біологічних наук Registration Date 06-12-2024 Organization Taras Shevchenko National University of Kyiv popup.description1 Characterization of the pathogenic microflora in the occurrence of purulent post-traumatic and post-surgical bacterial complications, as well as the selection, comprehensive characterization and testing of the antimicrobial effectiveness of broad-spectrum bacteriophages for the treatment of patients with bullet, mine-explosive wounds, and other injuries, to solve the problem of increasing antibiotic resistance, and the reconstruction of Ukraine in the war and post-war periods. popup.description2 Objects of the research: lytic bacteriophages specific to bacterial pathogens of bone-purulent pathologies relevant in Ukraine, and their properties. The aim of the work: isolation and study of the characteristics of lytic bacteriophages with potential activity against pathogenic bacteria. Methods of the research: biomaterial sampling, microbiological, biochemical, electron microscopy, bioinformatic and virologic research methods. A collection of 90 pure lines of bacteriophages of pathogenic bacteria Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii and Proteus mirabilis was created. The lytic activity spectra of the isolated phages ranged from 28-90%. Morphological studies showed that the isolated phages are representatives of myo-, podo- and siphoviruses. Whole-genome sequencing of two bacteriophages (Kr 29 and β1A) showed that these viruses do not have toxin or antibiotic resistance genes, and hence are promising antibacterial agents. The interaction of bacteriophages with antibiotics in vitro was studied and their synergistic effect was noted. A test system was developed to study the ability of bacteriophages to destroy bacterial biofilms on fomites. 100% inhibition of bacterial growth on the surface of PVC tubes after their immersion in a phage suspension was shown. It has been established that the storage temperature is a critical parameter for preserving the infectious properties of phage preparations. If the cold chain is not observed, the percentage of phage inactivation can reach 87.5%. Research into the effectiveness of phage mixtures against different types of bacteria has shown that single-component preparations or cocktails of bacteriophages against only one type of pathogenic bacteria are more effective. Product Description popup.authors Olena M. Andriichuk Iryna Н. Budzanivska Korniienko Nataliia O. Linenko Oleksandr M. Liutko Olha B. Moisa Ivan І. Pozhylov Iliia M. Poniatovskyi Vadym A. Snihur Halyna O. Kharina Alla V. popup.nrat_date 2024-12-06 Close