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Information × Registration Number 0224U032845, (0123U102789) , R & D reports Title New fluorescent dyes and multifluorophore systems for the improvement of sensitivity of flow cytofluorometry and fluorescence microscopy diagnostic methods popup.stage_title Дизайн, синтез та дослідження фотофізичних властивостей димерних барвників та FRET пар на основі протеїнів, а також мультифлуорофорних барвників на основі полімерів та дендримерів. Head Tatarets Anatolii L., Registration Date 09-12-2024 Organization State Scientific Institution Scientific and Technological Complex" Institute of Single Crystals "of the National Academy of Sciences of Ukraine popup.description1 Development of the principles for creating the new fluorescent materials with increased brightness and photostability to improve the sensitivity and robustness of diagnostics by flow cytometry and fluorescence microscopy. popup.description2  The main goal of the work is to develop principles of creating new fluorescent materials with increased brightness and photostability in order to improve sensitivity and reliability of diagnostics by flow cytometry and fluorescence microscopy methods. ‎ To achieve this goal, in the second stage of the project, a series of dimeric dyes based on acridine orange, 2,5-diaryloxazole derivatives, naphthalic acid derivatives, and cyanine dyes were synthesized. FRET pairs based on fluorescent proteins (R-phycoerythrin (R-PE) and peridinin-chlorophyll-protein complex (PerCP)) with cyanine and squaraine dyes were also obtained. For the dimeric dyes and FRET pairs, their spectral and luminescence characteristics were determined. Based on the developed methods of conjugation with biomolecules, we have synthesized conjugates of the monomeric fluorophores with polymeric compounds and biomolecules such as various proteins, hyaluronic acid, and dextrans of different molecular weight. The conjugates are multifluorophore systems. It is found that combination of a fluorescent dye with a rigid linker and/or dendron skeleton allows creating brighter fluorescent compounds, while use of flexible linkers usually leads to aggregation of dyes in aqueous solutions and quenching of their fluorescence. Linking of dyes through a short spacer also causes fluorescence quenching due to radiation-free resonance energy transfer. The hydrophilicity of a dye plays an important role in preventing aggregation and fluorescence quenching on a dendron or polymeric skeleton. The obtained patterns made it possible to develop dyes with increased brightness which are of great practical importance for use in biomedical research and clinical diagnostics by fluorescence microscopy and flow cytometry. Product Description popup.authors Vlasenko Hanna S. Kaidash Mariia B. Kryvoshei Oleksandr I. Obukhova Olena M. Posokhov Yevhen O. Prokopiuk Volodymyr Yu. Stepanenko Olena Yu. Tatarets Anatolii L. popup.nrat_date 2024-12-09 Close