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Information × Registration Number 0224U032946, (0124U003964) , R & D reports Title I-DRUG: The German Ukrainian Radiosensitizer Development Initiative popup.stage_title Отримання препаратів рекомбінантної аргінази та індоспіцину. Проведення пілотних досліджень на 2D-культурах на моделі клітин раку голови та шиї in vitro Head Stasyk Oleh V., Доктор біологічних наук Registration Date 12-12-2024 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description1 The vision of I-DRUG is to develop new innovative metabolic strategies that improve the curative potential of cancer radiotherapy. In particular, our goal is to investigate the radiosensitizing potential of combinational approaches of arginine-deprivation therapy (ADT) based on the sophisticated 3D spheroid assay platform and cellular models of head and neck squamous cell carcinomas (HNSCC). popup.description2  The goal of the I-DRUG project is to develop new innovative metabolic strategies that improve the therapeutic potential of radiation therapy for cancer, in particular, to study the radio sensitizing potential of enzymatic starvation for the amino acid arginine (ADT) in cell models of highly metastatic human head and neck squamous cell carcinomas (HNSCC). Our working hypothesis is the assumption that the anticancer efficacy of ADT can be improved by the rational design of combined approaches involving the recombinant arginine-degrading enzyme arginase (rhARG1) and certain additional chemotherapeutic factors, in particular plant-derived arginine analogues. Within the framework of the first stage of the project, a two-stage scheme for obtaining a purified rhARG1 drug based on constructed by us yeast producers was optimized, the advantages of which are high stability and efficiency as a component of ADT. Also, a unique cell model was created based on the subline of HNSCC SAS-R9 to study the mechanisms of radio sensitization, which is the main goal of the I-DRUG project. Extracts of the leguminous plant Indigofera spicata containing Arg analogues as components of combined ADT were obtained. All the obtained results will be a reliable basis for the successful implementation of the next stages of the project. Product Description popup.authors Vovk Olena I. Demash Dmytro V. Polishchuk Nikita V. Stasyk Oleh V. Stasyk Olena H. Shuvaieva Halyna Yu. popup.nrat_date 2024-12-12 Close