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Information × Registration Number 0224U033120, (0123U102912) , R & D reports Title Development of an effective antimicrobial and wound-healing biological preparation for prevention and treatment of purulent-inflammatory wounds of the skin and mucous membranes popup.stage_title Визначення ефективності застосування відібраних пробіотичних штамів, їх метаболітів та наночасток діоксиду церію (біопрепарату) для профілактики та лікування гнійних ран слизових оболонок за розроблених експериментальних моделей у тварин. Визначення ефективності застосування відібраних пробіотичних штамів та/або їх метаболітів та/або наночастинок діоксиду церію (біопрепарату) у комплексі з фітосептиками при запальних процесах слизової оболонки ротової порожнини. Head Spivak Mykola Ya., Доктор біологічних наук Registration Date 18-12-2024 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 Development of biological product based on probiotic Bacillus, Lactobacillus and Bifidobacterium strains with proven wound-healing, antimicrobial, anti-inflammatory and immunomodulatory properties and/or their metabolites and nanomaterials in order to introduce a personalized approach to prevention and treatment of purulent-inflammatory wounds of the skin and mucous membranes. popup.description2  Animal models of purulent wounds of mucous membranes of various genesis have been developed. The formation of infectious vulvovaginitis in mice caused by mechanical damage to the vulva mucosa and subsequent vaginal introduction of the Staphylococcus aureus strain B 918, ATCC 6538, was evidenced by the appearance of clinical signs of an infectious and inflammatory process against the background of a change in the vaginal microbiota with the dominance of opportunistic microorganisms and a decrease in the number of lactobacteria and bifidobacteria. Histological changes in the vaginal wall included significant swelling of the epithelial layer, its focal infiltration by polymorphonuclear leukocytes and lymphoid cells, as well as moderate infiltration by inflammatory cells of the submucosal layer, muscular and adventitia. The effectiveness of the prophylactic and therapeutic use of probiotic cultures separately or as part of various probiotic compositions, their metabolites, and cerium dioxide nanoparticles in experimental purulent wounds of the mucous membranes, their effect on the speed and efficiency of healing, elimination of the pathogen of the purulent process, and the state of the immune system in animals was determined. It was shown that the administration of probiotic compositions from the strains of bacilli – B. amyloliquefaciens ssp. plantarum IMV B-7142 – B. amyloliquefaciens ssp. plantarum IMV B-7143 or B. megaterium R1 - B. subtilis R7 – to mice with infectious vaginitis led to the normalization of the vaginal microbiota: the number of aerobic and facultatively anaerobic microorganisms decreased, primarily due to a decrease in the number of representatives of the genus Staphylococcus. Their therapeutic and immunomodulatory effect was evidenced by the gradual disappearance of external clinical signs of the infectious-inflammatory process, the activation of factors of the innate immunity (functional activity of cells of the phagocytic system) and the decrease Product Description popup.authors Babenko Lidiia P. Vasyliuk Olha M. Lazarenko Liudmyla M. Safronova Larysa A. Spivak Mykola Ya. popup.nrat_date 2024-12-18 Close