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Registration Number

0224U033152, (0123U100312) , R & D reports

Title

The study of the regenerative effects of multipotent stem cells at myocardial injury

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Встановлення регенеративних ефектів мультипотентних стовбурових клітин при пошкодженнях міокарда

Head

Kyryk Vitalii M., к.мед.н.

Registration Date

19-12-2024

Organization

State Institution "National Scientific Center "Institute of Cardiology, clinical and regenerative medicine named after academician M.D. Strazhesko" of the National Academy of Medical Sciences of Ukraine

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To establish the regenerative effects of multipotent stem cells in the case of myocardial damage

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For cultures of murine adipose tissue-derived multipotent mesenchymal stromal cells (MSCs), high proliferative activity and clonogenic potential were demonstrated, along with the expression of specific mesenchymal markers CD44, CD73, CD90, CD105, and the absence of expression of hematopoietic markers CD45 and CD117. These cells also showed the ability for directed osteogenic and adipogenic differentiation. Based on histological and electrocardiographic studies, a model of lipopolysaccharide (LPS)-induced inflammatory myocardial injury in mice was validated, and the optimal dose of E. coli LPS was determined to be 5 mg/kg, which resulted in more pronounced and sustained inflammatory changes compared to the 1 mg/kg dose. In the early stages following the transplantation of adipose tissue-derived MSCs in animals with induced inflammatory myocardial injury, regenerative effects were observed. These effects included a reduction in lipid dystrophy and myocardial fibrosis after inflammation, as evidenced by histological analysis, as well as improvements in disrupted cardiac excitability and conductivity functions, as shown by electrocardiographic studies. 

Product Description

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Vlasova Olena M.
Klymenko Pavlo P.
Rybachuk Oksana А.
Skybo Halyna H.
Ustymenko Alina M.
Tsupykov Oleh M.
Shmatko Halyna A.

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2024-12-19