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Information × Registration Number 0224U033212, (0124U003749) , R & D reports Title Functionalised carbon-based nanomaterials obtained by "green methods" from Ukrainian agricultural waste to combat multilevel neurotoxicity induced by xenobiotic/essential transition metals and arsenic. popup.stage_title Зелений синтез вуглецевих нанопористих матеріалів і наночастинок із качанів/стебел кукурудзи та сухих яблук, їх характеристика та визначення потенційної нейротоксичності та мембраноактивних властивостей. Head Krysanova Nataliya V., Кандидат біологічних наук Registration Date 21-12-2024 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description1 To synthesize/characterize/functionalize carbon-based nanoporous materials and nanoparticles from Ukrainian agricultural waste using green synthesis principles; to determine neurotoxicity risks of the nanomaterials and capacity to mitigate multilevel neurotoxicity induced by pollutant mixture of xenobiotic/essential transition heavy metals and arsenic (simultaneously preserving Ca-signalling) using own approach in brain nerve terminals; to confirm data using in vivo animal model. popup.description2 Approaches for green synthesis of carbon nanoporous materials and nanoparticles from corn cobs and dried apples were developed and their efficiency was tested. Carbon nanoporous materials and nanoparticles from corn cobs and dried apples were synthesized and characterized using TEM, FTIR, Raman spectroscopy and fluorimetry. Their size was determined and the presence of amino, hydroxyl and carboxyl/carbonyl groups on the surface of the particles was proven. The potential neurotoxicity and membrane-active properties of the synthesized materials and nanoparticles were analyzed using rat brain nerve terminals. It was found for the first time that the synthesized materials and nanoparticles did not have neurotoxic features and therefore they are biocompatible and can be used in biotechnology, medicine, environment technologies. An optimized model of metal-induced excitotoxicity using a radioisotope method for analyzing neurotransmitter transport in rat brain nerve terminals was applied to investigate the ability of carbon nanoporous materials and nanoparticles to reduce the neurotoxic effects of heavy metals. It was first shown using various methods, namely, monitoring the transport of radioactively labeled excitatory and inhibitory neurotransmitters L-[14C] glutamate and [3H] GABA and fluorescent methods, the ability carbon nanoporous materials and nanoparticles synthesized by pyrolysis from corn cobs and dried apples to chelate heavy metals Hg, Pb and Cd in a biological system. It has been shown for the first time that nanoporous materials and nanoparticles obtained by hydrothermal carbonization have different neuroactive properties compared to nanoporous materials and nanoparticles synthesized by pyrolysis. Based on the results, one paper was published and methodological recommendations were developed in 2024 with a single reference to the project.   Product Description popup.authors Ivanytsia Mykyta O. Borysov Arsenii A. Pozdniakova Nataliia H. Tarasenko Alla S Terebilenko Anastasiia V. popup.nrat_date 2024-12-21 Close