Search academic texts

Information × Registration Number 0224U033266, (0122U000659) , R & D reports Title Construction of biosensors and biofuel cells based on a combination of microbial cells - producers of oxidoreductases, purified enzymes and nanocomposite materials popup.stage_title Створення лабораторних прототипів біопаливних комірок на основі мікробних клітин – надпродуцентів оксидоредуктаз, очищених ферментів та нанозимів і/або наномедіаторів. Head Honchar Mykhailo V., д.б.н. Registration Date 23-12-2024 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description1 Creation of compositions based on microbial cells - overproducers of oxidoreductases, purified enzymes and new nanomaterials with nanozyme or nanomediatorial activity and their functional testing as part of biosensors and biofuel elements. popup.description2 The immobilization of synthesized nanoparticles and purified enzymes on biofuel cells’ electrodes was carried out and functional characteristics of bionanoelectrodes were studied. Using new catalytically active nanomaterials (nanozymes and electron transfer nanomediators) as well as purified microbial oxidoreductases and hydrolases, laboratory prototypes of enzymatic biofuel cells (BFC) were created. A microbial biofuel cell was constructed using creatinine as an energy substrate and recombinant cells of Corynebacterium glutamicum (an overproducer of creatinine deiminase), as a bioanode catalytic component. For the first time, an enzymatic BFC with an anode, based on flavocytochrome b2 of Ogataea polymorpha bound with nPtAu as a nanomediator, as well with a cathode, based on laccase bound with the nanomediator nAuCePt, was developed. This constructed BFC exhibits a specific power of 1.1 μW/cm², which significantly exceeds the power of the tested variants with using other nanomediators. Laboratory prototypes of a BFC with a bioanode, based on O. polymorpha alcohol oxidase, and a cathode with fungal laccase were created using various nanomaterials to immobilize enzymes, improve the efficiency of electron transfer and increase the stability of the fuel cell.Enzyme bioanodes were created based on various purified enzymes: recombinant arginine deiminase of Mycoplasma hominis (ADI), recombinant human arginase I in combination with urease, or arginine oxidase from Amanita phalloides (ArgO), conjugated with nCdCu nanoparticles. A microbial bioanode was created based on recombinant Corynebacterium glutamicum cells, additionally enriched with creatine deiminase (CDI-nCu bionanocomposite), which was used in the construction of a two-chamber BFC for energy generation from creatinine present in blood serum and urine. Laccase from the tinder fungus Trametes zonata was used as a biocathode. The constructed microbial biofuel cell was tested on real human urine and serum samples Product Description popup.authors Haida Halyna Z. Horecha Mariia V. Demkiv Olha M. Zakalska Oksana M. Zakalskyi Andrii Ye. Naum Yuliia Ye. Serkiz Roman Ya. Stasiuk Nataliia Ye. popup.nrat_date 2024-12-23 Close