Search academic texts

Information × Registration Number 0224U033283, (0122U002240) , R & D reports Title The role of immunomodulatory in the antitumor action of traditional and experimental medicines at their delivery by polymeric nanocarriers to various target cells in vitro and in vivo popup.stage_title З’ясування механізмів перехресної резистентності пухлинних клітин ссавців до дії гетероциклічних сполук і традиційних протипухлинних препаратів. Дослідження впливу цих сполук на імунокомпетентні клітини у мишей з експериментальними пухлинами Head Stoika Rostyslav S., д.б.н. Registration Date 24-12-2024 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description1 The aim of the project is to investigate the role of immunomodulatory in the antitumor action of traditional and experimental medicines in free form and in complexes with synthetic and polymeric nanovectors. popup.description2  As part of the 1st stage of the work, the time-dependent effect of new hybrid derivatives of thiopyrano[2,3-d]thiazole with 5-hydroxy-1,4-naphthoquinone (compounds Les-6547 and Les-6557) on DNA biosynthesis and damage was investigated. Studied compounds affected distribution of cell cycle stages, elevated the content of reactive oxygen species, apoptosis induction indicators (caspase 3/7, 8, 9, 10 activity), and reduced mitochondrial membrane potential in cells of different lines (HCT116, HCT116 p53-/-, SW480, DLD-1, HT-29) of human colorectal cancer cells. Within the 2nd stage of the work, the use of individual semicarbazones (concentration and duration of action) as potential inducers of immunological cell death upon re-inoculation of an experimental tumor (melanoma B16F10/wt) into laboratory mice was optimized. The lifespan of animals that were subjected to immunization and the size of their tumors after re-inoculation of the tumor were determined. Within the 3rd stage of the work, the dimeric form of the synthetic tripeptide Ser-Pro-Cys (SPC) was synthesized. This tripeptide was previously discovered by the authors in the blood serum of patients with autoimmune diseases. The effect of monomeric and dimeric forms of this tripeptide on the viability of pseudonormal and tumor cells of animals and humans in vitro was compared. As part of the 4th stage of the work, it was shown that the drug created by the department based on chitosan hydrogel containing hyaluronic acid and cannabimimetic N-stearoylethanolamine effectively treats superficial wounds in laboratory mice. An effective wound-healing fatty drug of chitosan containing the antibiotic ampicillin, vegetable (linseed) oil and N-stearoylethanolamine was also created. Scanning electron microscopy revealed a porous surface structure of both wound-healing drugs, which promotes their adhesion to the wound surface. Product Description popup.authors Ivasechko Iryna I. Antoniuk Volodymyr O. Kashchak Nataliia I. Klishch Mykola V. Kliuchivska Olha Yu. Kozak Yuliia S. Lutsyk Maksym D. Manko Nazar O. Panchuk Rostyslav R. Senkiv Yuliia V. Skorokhid Nadiia R. Starykovych Maryna O. Stoika Rostyslav S. Finiuk Nataliia S. popup.nrat_date 2024-12-24 Close